| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | DEN1; NEDP1; PRSC2 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于SENP8抗体的3篇参考文献的简要概括(注：SENP8可能指NEDD8特异性蛋白酶，相关研究较少，以下为模拟示例)：
---
1. **标题**：*Structural basis for NEDD8 recognition and cleavage by SENP8*
**作者**：Mendoza HM et al.
**摘要**：该研究解析了SENP8蛋白酶的晶体结构，揭示其特异性识别并切割NEDD8(一种泛素样蛋白)的分子机制。抗体被用于免疫沉淀实验验证SENP8与底物的相互作用。
2. **标题**：*SENP8 regulates neuronal development via modulating neddylation pathways*
**作者**：Li Y et al.
**摘要**：通过SENP8抗体进行Western blot和免疫荧光实验，发现SENP8通过调控NEDD8去修饰影响神经突触形成，提示其在神经退行性疾病中的潜在作用。
3. **标题**：*Development of a monoclonal antibody specific for SENP8 and its application in cancer biomarker screening*
**作者**：Wang Q et al.
**摘要**：报道了一种高特异性SENP8单克隆抗体的开发，并通过组织芯片分析显示SENP8在多种肿瘤中异常表达，可能作为癌症诊断的生物标志物。
---
**注意**：以上文献为模拟内容，实际研究中SENP8相关抗体文献较少，建议通过PubMed或Google Scholar以“SENP8 antibody”或“NEDD8 protease DEN1”等关键词检索最新数据。
SENP8. also known as DEN1/NEDP1. is a member of the sentrin/SUMO-specific protease (SENP) family, which regulates post-translational protein modification by cleaving ubiquitin-like modifiers. Unlike most SENPs that primarily process small ubiquitin-like modifier (SUMO) proteins, SENP8 specifically hydrolyzes the ubiquitin-like protein NEDD8 (neural precursor cell expressed, developmentally downregulated 8) from conjugated substrates. This activity is critical for modulating the neddylation pathway, a process analogous to ubiquitination that regulates protein stability, localization, and function. SENP8-mediated deneddylation counteracts the activity of NEDD8-conjugating enzymes, thereby influencing key cellular processes such as cell cycle progression, DNA repair, and stress responses. Notably, SENP8 plays a regulatory role in the Cullin-RING ubiquitin ligase (CRL) family by removing NEDD8 from Cullin proteins, which is essential for maintaining CRL activity and substrate turnover.
Antibodies targeting SENP8 are vital tools for studying its expression, localization, and function in biological systems. They enable detection of SENP8 in immunoassays (e.g., Western blot, immunoprecipitation) and visualization in cellular or tissue contexts (e.g., immunofluorescence, immunohistochemistry). Research using SENP8 antibodies has elucidated its involvement in diseases such as cancer, where dysregulated neddylation contributes to tumorigenesis, and neurodegenerative disorders linked to protein aggregation. These antibodies also aid in exploring SENP8’s crosstalk with other post-translational modification pathways, including ubiquitination and SUMOylation. Due to SENP8’s specificity for NEDD8. its antibodies are distinct from those targeting SUMO-directed SENPs, emphasizing the need for rigorous validation to ensure specificity in experimental models.
×
