| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | DCE; SELADIN1; Nbla03646; seladin-1 |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DHCR24 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DHCR24抗体的3篇代表性文献，涵盖其在不同研究中的应用和功能分析：
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1. **文献名称**: *DHCR24 contributes to the malignant phenotype of hepatocellular carcinoma via modulating ROS homeostasis*
**作者**: Li X, et al.
**摘要**: 该研究利用DHCR24抗体检测肝癌细胞中DHCR24蛋白的表达水平，发现其高表达与肿瘤增殖和氧化应激抵抗相关。通过敲低DHCR24可增加活性氧(ROS)水平并抑制肝癌生长，提示其作为潜在治疗靶点。
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2. **文献名称**: *DHCR24-mediated cholesterol synthesis in Alzheimer's disease: A proteomic analysis*
**作者**: Grimm MO, et al.
**摘要**: 研究采用DHCR24抗体进行免疫印迹和免疫组化，发现阿尔茨海默病患者脑组织中DHCR24表达降低，导致胆固醇代谢异常和β-淀粉样蛋白沉积增加，提示其在神经退行性疾病中的保护作用。
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3. **文献名称**: *DHCR24 regulates lipid droplet accumulation in macrophages during atherosclerosis*
**作者**: Zhang Y, et al.
**摘要**: 通过DHCR24抗体检测巨噬细胞中该酶的表达，发现其通过调控胆固醇酯合成影响脂滴形成，促进动脉粥样硬化斑块发展。抑制DHCR24可减少泡沫细胞形成，为心血管疾病提供新机制。
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这些文献展示了DHCR24抗体在癌症、神经退行性疾病和心血管疾病研究中的关键作用，涉及蛋白功能验证、病理机制探索及治疗潜力分析。
The DHCR24 antibody targets the enzyme 3β-hydroxysterol Δ24-reductase (DHCR24), a key player in cholesterol biosynthesis. This enzyme catalyzes the reduction of desmosterol to cholesterol, the final step in the Bloch pathway of cholesterol synthesis. Beyond its role in lipid metabolism, DHCR24 is implicated in cellular stress responses, oxidative defense, and apoptosis regulation. It is also known as Seladin-1 (Selective Alzheimer's Disease Indicator-1), as its downregulation has been linked to Alzheimer's disease pathology, potentially through disrupted cholesterol homeostasis and increased neuronal vulnerability.
DHCR24 antibodies are widely used in research to study the enzyme's expression, localization, and function in various tissues and disease models. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into DHCR24's roles in cancer, neurodegeneration, and metabolic disorders. For instance, DHCR24 overexpression has been observed in certain cancers, correlating with tumor progression and therapy resistance, while its deficiency is associated with developmental defects and lipid metabolism disorders.
The antibody's specificity and reliability are critical for elucidating DHCR24's dual roles as a cholesterol synthase and a stress-response mediator, offering insights into therapeutic strategies targeting cholesterol-related diseases.
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