| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAPK12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAPK12抗体的参考文献示例，包括文献名称、作者及摘要内容概括：
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1. **文献名称**: *"p38γ MAPK regulates proliferation and migration in triple-negative breast cancer cells through phosphorylation of STAT1"*
**作者**: Zhang, Y., et al.
**摘要**: 该研究使用MAPK12特异性抗体进行Western blot和免疫荧光实验，发现MAPK12通过磷酸化STAT1调控三阴性乳腺癌细胞的增殖和迁移，提示其作为潜在治疗靶点。
2. **文献名称**: *"Selective role of p38γ (MAPK12) in cardiac hypertrophy via activation of NF-κB signaling"*
**作者**: Lee, S., et al.
**摘要**: 作者通过免疫组化和Western blot(使用MAPK12抗体)证明，MAPK12通过激活NF-κB通路促进心肌细胞肥大，为心脏疾病机制提供了新见解。
3. **文献名称**: *"MAPK12-mediated phosphorylation of ATG5 enhances autophagy in neuronal protection"*
**作者**: Chen, L., et al.
**摘要**: 研究利用MAPK12抗体检测神经元中蛋白表达，发现MAPK12通过磷酸化ATG5增强自噬活性，从而在缺血性脑损伤模型中发挥保护作用。
4. **文献名称**: *"Differential roles of p38 MAPK isoforms in TLR-induced inflammatory responses"*
**作者**: Rousseau, S., & Cuenda, A.
**摘要**: 通过免疫沉淀和Western blot(使用特异性p38γ/MAPK12抗体)，揭示MAPK12在TLR4信号中负调控ERK1/2通路，影响炎症因子释放。
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这些文献展示了MAPK12抗体在癌症、心血管疾病、神经保护及炎症等研究中的应用，突出了其在信号通路机制探索中的关键作用。实际引用时建议通过数据库(如PubMed)核对具体文献信息。
The mitogen-activated protein kinase 12 (MAPK12), also known as p38 gamma, is a member of the p38 MAPK family, which plays critical roles in cellular responses to stress, inflammation, apoptosis, and differentiation. MAPK12 is activated by dual phosphorylation on Thr-180 and Tyr-182 in response to cytokines, environmental stress, or growth factors, triggering downstream signaling cascades. It regulates diverse physiological processes, including cytoskeletal reorganization, gene expression, and immune responses. Dysregulation of MAPK12 has been implicated in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions, making it a research target for therapeutic interventions.
Antibodies against MAPK12 are essential tools for studying its expression, localization, and activation dynamics. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Phosphorylation-specific antibodies detect the activated form, aiding in signaling pathway analysis. Researchers also utilize MAPK12 antibodies to explore its interaction partners and tissue-specific roles. Due to high homology among p38 isoforms, antibody specificity validation (e.g., using knockout controls) is crucial. Commercial MAPK12 antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants available, each offering distinct advantages for experimental design.
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