| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | FP; PGL5; SDH1; SDH2; SDHF; CMD1GG |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SDHA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SDHA抗体的参考文献，涵盖其应用与机制研究：
1. **《SDHA mutations in cancer》**
- 作者：Bardella, C., et al.
- 摘要：研究探讨SDHA基因突变与多种癌症的关联，强调SDHA抗体在肿瘤组织免疫组化中的诊断价值，可用于识别SDH复合体功能异常相关的肿瘤(如胃肠道间质瘤和嗜铬细胞瘤)。
2. **《Immunohistochemistry for SDHB divides genetic from nongenetic cases of pheochromocytoma/paraganglioma》**
- 作者：van Nederveen, F.H., et al.
- 摘要：文章验证SDHA/SDHB抗体在副神经节瘤和嗜铬细胞瘤中的诊断效用，指出SDHA表达缺失可作为遗传性SDH复合体缺陷的标志，辅助临床分型与遗传咨询。
3. **《The role of SDHAF2 in syndromic paraganglioma》**
- 作者：Hao, H.X., et al.
- 摘要：通过分析SDHAF2突变对SDHA蛋白稳定性的影响，研究显示SDHA抗体可用于检测SDH组装因子的功能异常，为遗传性副神经节瘤的分子机制提供依据。
以上文献均聚焦于SDHA抗体在肿瘤病理诊断及遗传性疾病研究中的应用。
The SDHA antibody targets the succinate dehydrogenase complex flavoprotein subunit A (SDHA), a key component of mitochondrial complex II involved in both the tricarboxylic acid (TCA) cycle and electron transport chain. SDHA catalyzes the oxidation of succinate to fumarate, linking energy production pathways. As a housekeeping protein, SDHA is ubiquitously expressed, making it a common loading control in Western blotting. However, its role extends beyond metabolic regulation; SDHA germline mutations are associated with hereditary paraganglioma-pheochromocytoma syndromes, gastrointestinal stromal tumors (GISTs), and mitochondrial disorders.
SDHA antibodies are widely used in research and diagnostics to assess SDHA protein expression, particularly in identifying SDHA-deficient tumors caused by pathogenic variants. Loss of SDHA expression detected via immunohistochemistry (IHC) often signals underlying genetic mutations, guiding genetic testing and clinical management. Additionally, these antibodies aid in studying mitochondrial dysfunction mechanisms in neurodegenerative diseases and cancer metabolism. Commercial SDHA antibodies are typically validated for specificity across applications like immunofluorescence, IHC, and immunoblotting. Recent studies also explore SDHA's role in reactive oxygen species regulation, expanding its relevance to oxidative stress-related pathologies. Proper validation remains critical due to potential cross-reactivity with other flavoproteins or tissue-specific isoforms.
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