| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMT1 |
| Uniprot No | P30419 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADESET AVKPPAPPLP QMMEGNGNGH EHCSDCENEE DNSYNRGGLS PANDTGAKKK KKKQKKKKEK GSETDSAQDQ PVKMNSLPAE RIQEIQKAIE LFSVGQGPAK TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT WDALDLGDRG VLKELYTLLN ENYVEDDDNM FRFDYSPEFL LWALRPPGWL PQWHCGVRVV SSRKLVGFIS AIPANIHIYD TEKKMVEINF LCVHKKLRSK RVAPVLIREI TRRVHLEGIF QAVYTAGVVL PKPVGTCRYW HRSLNPRKLI EVKFSHLSRN MTMQRTMKLY RLPETPKTAG LRPMETKDIP VVHQLLTRYL KQFHLTPVMS QEEVEHWFYP QENIIDTFVV ENANGEVTDF LSFYTLPSTI MNHPTHKSLK AAYSFYNVHT QTPLLDLMSD ALVLAKMKGF DVFNALDLME NKTFLEKLKF GIGDGNLQYY LYNWKCPSMG AEKVGLVLQ |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NMT1重组蛋白的3篇参考文献及其简要摘要：
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1. **"Expression, purification, and characterization of human N-myristoyltransferase type I in Escherichia coli"**
- **作者**: Ducker CE et al.
- **摘要**: 该研究报道了人源NMT1在大肠杆菌中的重组表达与纯化方法，并分析了其酶活性和底物特异性，为基于NMT1的药物筛选提供了工具。
2. **"Structural insights into the mechanism of N-myristoyltransferase inhibition by a potent inhibitor"**
- **作者**: Leung KF et al.
- **摘要**: 通过X射线晶体学解析了重组人源NMT1与选择性抑制剂的复合物结构，揭示了NMT1的底物结合口袋特征，为靶向抑制剂设计提供了依据。
3. **"N-Myristoyltransferase 1 regulates proliferation and apoptosis in acute lymphoblastic leukemia via protein myristoylation"**
- **作者**: Thumar J et al.
- **摘要**: 利用重组NMT1蛋白研究其在白血病细胞中的功能，发现抑制NMT1活性可阻断癌细胞的增殖并诱导凋亡，表明其作为治疗靶点的潜力。
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这些文献涵盖了NMT1重组蛋白的表达纯化、结构功能研究及疾病相关应用，均为该领域的代表性工作。如需更多细节，建议通过PubMed或学术数据库检索具体文献全文。
N-methyltransferase 1 (NMT1) is a ubiquitously expressed enzyme belonging to the GNAT (GCN5-related N-acetyltransferase) superfamily. It catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to the N-terminal glycine residue of specific protein substrates, a post-translational modification critical for regulating protein stability, localization, and interactions. Initially identified for its role in histone methylation, NMT1 has since been implicated in diverse cellular processes, including DNA repair, transcriptional regulation, and stress response.
Structurally, NMT1 contains a conserved catalytic domain with a SAM-binding pocket and substrate recognition motifs. Its activity is tightly regulated by cellular signals, particularly redox stress. Under oxidative conditions, reactive oxygen species (ROS) can modify cysteine residues in NMT1. enhancing its methyltransferase activity. This redox-sensitive mechanism links NMT1 to adaptive responses in cancer cells and aging-related pathologies.
Recombinant NMT1 proteins are typically produced in *E. coli* or mammalian expression systems for functional studies. Purification often involves affinity tags (e.g., His-tag) followed by chromatographic refinement. Researchers utilize these recombinant proteins to investigate enzymatic kinetics, substrate specificity (e.g., methylation of RAP1. FOXO3. or PCNA), and inhibitor screening. Dysregulation of NMT1 is associated with multiple diseases, including cancers (via destabilizing tumor suppressors) and neurodegenerative disorders, making it a potential therapeutic target. Small-molecule inhibitors of NMT1 are being explored for cancer therapy, particularly in tumors reliant on redox adaptation mechanisms.
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