| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | TAF2J; TAFII20 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TAF12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TAF12抗体的3篇参考文献的简要总结(注：内容基于模拟的典型研究场景，具体文献需通过学术数据库核实)：
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1. **文献名称**：*TAF12 is essential for the recruitment of TFIID to core promoters*
**作者**：Wright, K.J., et al.
**摘要**：本研究利用TAF12特异性抗体进行染色质免疫沉淀(ChIP)和免疫共沉淀(Co-IP)，揭示了TAF12在TFIID复合体组装中的关键作用，证明其缺失会破坏RNA聚合酶II在启动子区域的招募，影响转录起始。
2. **文献名称**：*Structural insights into the human TFIID complex through cryo-EM*
**作者**：Papai, G., et al.
**摘要**：通过冷冻电镜解析人源TFIID复合体的高分辨率结构，结合TAF12抗体的免疫印迹(WB)验证亚基组成，阐明了TAF12与其他TAF蛋白的相互作用界面，为转录调控机制提供结构基础。
3. **文献名称**：*TAF12 dysregulation promotes oncogenic transformation in hepatocellular carcinoma*
**作者**：Chen, Y., et al.
**摘要**：采用TAF12抗体进行免疫组化(IHC)和Western blot分析，发现TAF12在肝癌组织中异常高表达，并通过调控细胞周期相关基因促进肿瘤增殖，提示其作为潜在治疗靶点。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“TAF12 antibody”、“TFIID TAF12”等获取最新研究。
The TATA-box binding protein-associated factor 12 (TAF12) is a critical subunit of the TFIID complex, a general transcription factor essential for RNA polymerase II-mediated gene transcription. As part of the TFIID complex, TAF12 interacts with TBP (TATA-box binding protein) and other TAFs to facilitate promoter recognition, chromatin remodeling, and assembly of the pre-initiation complex. TAF12 contains a conserved histone-fold domain that enables dimerization with TAF6. forming a structural scaffold for TFIID. Beyond TFIID, TAF12 is also found in other transcriptional coactivator complexes, such as STAGA and TFTC, highlighting its versatile role in regulating gene expression.
TAF12 antibodies are widely used in research to investigate transcriptional mechanisms, protein-protein interactions, and chromatin dynamics. They are essential tools in techniques like chromatin immunoprecipitation (ChIP), co-immunoprecipitation (Co-IP), and Western blotting to study TAF12’s localization, binding partners, and expression levels. Dysregulation of TAF12 has been implicated in cancers and developmental disorders due to its role in controlling cell cycle and differentiation-related genes. For example, TAF12 overexpression or mutations may disrupt transcriptional fidelity, contributing to oncogenesis. Researchers also utilize TAF12 antibodies to explore its crosstalk with histone acetyltransferases and other epigenetic modifiers, providing insights into disease pathways and potential therapeutic targets. Its molecular weight (~20-25 kDa) varies slightly across species due to post-translational modifications.
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