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Rabbit Polyclonal TCF3 Antibody

  • 中文名: TCF3抗体
  • 别    名: E2A; E47; p75; AGM8; ITF1; VDIR; TCF-3; bHLHb21
货号: IPDX08846
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/250-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesE2A; E47; p75; AGM8; ITF1; VDIR; TCF-3; bHLHb21
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenFusion protein of human TCF3
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是3-4条关于TCF3抗体的参考文献示例(内容为虚构,仅供参考格式):

1. **文献名称**: "TCF3 expression profiling in B-cell malignancies using a novel monoclonal antibody"

**作者**: Smith A, et al.

**摘要**: 本研究开发了一种特异性识别TCF3蛋白的单克隆抗体,并通过免疫沉淀和免疫荧光验证其可靠性。研究发现,TCF3在B细胞淋巴瘤中高表达,并与患者预后不良相关。

2. **文献名称**: "Role of TCF3 in acute lymphoblastic leukemia: Insights from antibody-based detection"

**作者**: Chen L, et al.

**摘要**: 通过Western blot和流式细胞术,利用TCF3抗体分析白血病细胞系,发现TCF3的异常表达与NOTCH1信号通路失调相关,提示其作为潜在治疗靶点。

3. **文献名称**: "Antibody-mediated inhibition of TCF3/E2A dimerization in transcriptional regulation"

**作者**: Gupta R, et al.

**摘要**: 采用TCF3特异性抗体阻断其与E蛋白的二聚化,证实TCF3在调控B细胞分化相关基因(如EBF1和PAX5)中的关键作用,为免疫疾病机制研究提供新方向。

4. **文献名称**: "TCF3 antibody application in developmental biology: Insights from mouse models"

**作者**: Tanaka K, et al.

**摘要**: 通过免疫组化结合TCF3抗体,系统分析了小鼠胚胎发育中TCF3的时空表达模式,揭示其在神经嵴细胞迁移和器官形成中的动态调控。

注:以上文献为示例,实际引用需根据真实研究补充。建议通过PubMed或Google Scholar检索关键词“TCF3 antibody”或“TCF3 E2A”获取具体文献。

背景信息

TCF3 (Transcription Factor 3), also known as E2A, is a helix-loop-helix (HLH) transcription factor encoded by the TCF3 gene. It plays critical roles in regulating gene expression during cellular differentiation, particularly in early B-cell and T-cell development. TCF3 binds to E-box DNA motifs (CANNTG) to activate or repress target genes, often partnering with tissue-specific regulators like EBF1 or ID proteins to modulate lymphoid lineage commitment. Its function is essential for V(D)J recombination, a process that generates diverse antibody repertoires in adaptive immunity. Dysregulation of TCF3 is linked to hematological malignancies, including pre-B-cell acute lymphoblastic leukemia (ALL), where chromosomal translocations (e.g., TCF3-PBX1) result in oncogenic fusion proteins. TCF3 antibodies are widely used in research to study its expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence. These antibodies help elucidate TCF3's role in normal immune cell maturation, its crosstalk with signaling pathways (e.g., Wnt/β-catenin), and its involvement in diseases. Additionally, TCF3 is implicated in maintaining pluripotency in embryonic stem cells, expanding its relevance to regenerative medicine and cancer biology studies.

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