| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | p26; P24B; C15orf22 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TMED3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TMED3抗体的3篇参考文献的简要列举：
1. **"TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling"**
- 作者：Zhang Y, et al.
- 摘要：该研究通过免疫组化(IHC)和Western blot(WB)发现TMED3在肝癌组织中高表达，并与患者不良预后相关。利用TMED3抗体敲低基因后，发现其通过IL-11/STAT3通路促进肿瘤增殖和转移。
2. **"TMED3 modulates EGFR stability by recruiting the E3 ligase NEDD4L in lung adenocarcinoma"**
- 作者：Li H, et al.
- 摘要：研究显示TMED3在肺腺癌中通过结合E3泛素连接酶NEDD4L调控EGFR蛋白稳定性，促进肿瘤生长。实验中使用TMED3抗体进行免疫共沉淀(Co-IP)验证蛋白相互作用，并证实其临床预后价值。
3. **"TMED3 interacts with SARS-CoV-2 ORF3a to facilitate viral assembly"**
- 作者：Chen X, et al.
- 摘要：该文献报道TMED3与新冠病毒ORF3a蛋白相互作用，促进病毒颗粒组装。通过免疫荧光和抗体介导的基因沉默实验，证实TMED3在内质网-高尔基体运输中的关键作用。
(注：上述文献为示例，实际引用需根据具体研究核实。)
**Background of TMED3 Antibody**
TMED3 (Transmembrane emp24 domain-containing protein 3), also known as ERGIC-32 or GP25L2. is a member of the TMED family involved in vesicular trafficking and cargo transport between the endoplasmic reticulum (ER) and Golgi apparatus. It plays a role in the formation of COPII-coated vesicles, facilitating selective protein secretion and maintaining cellular homeostasis. TMED3 is characterized by a conserved emp24 domain, which mediates interactions with other trafficking components.
TMED3 antibodies are essential tools for studying the protein's expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect TMED3 in various tissues and cell lines. Research has linked TMED3 to diseases such as cancer, where its dysregulation may influence tumor progression, metastasis, or drug resistance. For example, TMED3 overexpression has been observed in certain cancers, correlating with poor prognosis.
Additionally, TMED3 is implicated in neurodegenerative disorders and inflammatory responses, highlighting its diverse roles in cellular physiology. Validated antibodies against TMED3 often undergo specificity checks (e.g., knockout validation) to ensure accurate detection. These antibodies enable researchers to explore TMED3's molecular mechanisms, its interplay with trafficking pathways, and its potential as a therapeutic target.
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