| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HsHPK; MRD57; PKU-ALPHA |
| WB Predicted band size | 88 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TLK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TLK2抗体的3篇参考文献概览(注：文献为示例性内容，实际引用需核实)：
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1. **文献名称**：*TLK2 kinase activity is critical for prostate cancer progression and metastasis*
**作者**：Smith A, et al.
**摘要**：研究通过开发特异性TLK2抗体，验证其在前列腺癌组织中的高表达，并揭示TLK2通过调控细胞周期蛋白促进肿瘤转移的机制。抗体用于免疫组化及Western blot分析，证实TLK2活性与患者预后相关。
2. **文献名称**：*Development of a monoclonal antibody targeting the catalytic domain of TLK2 for functional studies*
**作者**：Chen L, et al.
**摘要**：本文报道了一种靶向TLK2激酶结构域的单克隆抗体制备方法。该抗体成功应用于免疫沉淀和体外激酶活性检测，为研究TLK2在DNA损伤修复中的功能提供了工具。
3. **文献名称**：*TLK2 inhibition sensitizes glioblastoma cells to radiation therapy via impaired DNA repair*
**作者**：Wang Y, et al.
**摘要**：研究利用TLK2特异性抗体(克隆号：AB-T2-3C)验证其在胶质母细胞瘤中的表达，并通过抗体介导的功能抑制实验，证明TLK2失活可增强放疗敏感性，机制与同源重组修复缺陷相关。
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如需具体文献，建议在PubMed或Web of Science以“TLK2 antibody”为关键词检索，并筛选涉及抗体开发、验证或功能应用的论文。
The Tousled-like kinase 2 (TLK2) is a serine/threonine kinase belonging to the Tousled-like kinase (TLK) family, which shares homology with the Arabidopsis Tousled protein involved in development. TLK2. along with its paralog TLK1. plays critical roles in DNA repair, cell cycle regulation, and chromatin remodeling. It phosphorylates downstream targets such as the histone chaperone ASF1A and histone H3 (HIST1H3A), facilitating chromatin assembly during DNA replication and damage response. TLK2 is ubiquitously expressed but shows higher levels in tissues like testes, thymus, and colon, with its activity modulated by stress signals, including DNA damage and oxidative stress.
TLK2-specific antibodies are essential tools for studying its expression, localization, and function. These antibodies enable detection of TLK2 in techniques like Western blotting, immunoprecipitation, and immunohistochemistry. Dysregulation of TLK2 has been implicated in cancer, with studies linking its overexpression or inactivation to tumor progression, therapy resistance, and poor prognosis. For example, TLK2 inhibition sensitizes cancer cells to genotoxic therapies, highlighting its potential as a therapeutic target. However, conflicting reports on TLK2's role in different cancer types underscore the need for context-specific research. Commercial and custom-generated TLK2 antibodies vary in specificity, requiring validation via knockout controls or siRNA knockdown. Ongoing research aims to clarify TLK2's interplay with signaling pathways and its utility as a biomarker or drug target in precision oncology.
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