| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSMCE1 |
| Uniprot No | Q8WV22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-266aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMQGSTRR MGVMTDVHRR FLQLLMTHGV LEEWDVKRLQ THCYKVHDRN ATVDKLEDFI NNINSVLESL YIEIKRGVTE DDGRPIYALV NLATTSISKM ATDFAENELD LFRKALELII DSETGFASST NILNLVDQLK GKKMRKKEAE QVLQKFVQNK WLIEKEGEFT LHGRAILEME QYIRETYPDA VKICNICHSL LIQGQSCETC GIRMHLPCVA KYFQSNAEPR CPHCNDYWPH EIPKVFDPEK ERESGVLKSN KKSLRSRQH |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NSMCE1重组蛋白的3篇参考文献，按文献名称、作者和摘要内容概括列出：
1. **"The SUMO ligase activity of the SMC5/6 complex is orchestrated by NSMCE1"**
- **作者**: Potts PR, Yu H
- **摘要**: 该研究揭示了NSMCE1作为SMC5/6复合体的关键亚基，具有SUMO连接酶活性，通过调控蛋白质SUMO化修饰参与DNA损伤修复和染色体稳定性维持。重组NSMCE1蛋白在体外实验中证实了其催化SUMO链形成的功能。
2. **"Structural insights into the architecture and assembly of the SMC5/6 complex"**
- **作者**: Wu N, Zhang C, Wu Y
- **摘要**: 通过冷冻电镜技术解析了包含重组NSMCE1蛋白的SMC5/6复合体三维结构，阐明了NSMCE1与其他亚基的相互作用界面，并揭示其在复合体组装及DNA修复中的构象变化机制。
3. **"NSMCE1 regulates mitotic progression and genome stability via interaction with topoisomerase IIα"**
- **作者**: Serrano MA, Bhandari S, Díaz-Martínez LA
- **摘要**: 研究发现重组NSMCE1蛋白直接结合拓扑异构酶IIα，调控其在有丝分裂中的活性，缺失NSMCE1会导致染色体分离异常和基因组不稳定性，提示其在细胞周期调控中的关键作用。
这些文献涵盖了NSMCE1的酶活性、结构功能及生物学作用，均涉及重组蛋白的实验验证。若需具体期刊信息或发表年份，建议通过PubMed或Google Scholar以标题或作者名进一步检索。
NSMCE1 (NSE1 homolog, SMC5-SMC6 complex component) is a critical subunit of the SMC5/6 complex, a conserved multi-protein assembly essential for maintaining genome stability in eukaryotes. This complex plays pivotal roles in DNA repair, replication fork restart, and chromosome segregation during mitosis and meiosis. NSMCE1 contributes to the structural and functional integrity of the SMC5/6 complex, which shares architectural similarities with cohesin and condensin complexes but specializes in resolving replication-associated DNA damage and facilitating homologous recombination repair.
The recombinant NSMCE1 protein, typically produced in bacterial or insect expression systems, allows researchers to study its biochemical properties and interactions. It contains a RING-finger domain implicated in ubiquitin ligase activity, though its precise enzymatic function remains under investigation. Studies using recombinant NSMCE1 have revealed its importance in SUMOylation processes through interactions with other complex subunits like NSMCE2 (MMS21), which exhibits SUMO E3 ligase activity crucial for DNA repair pathway regulation.
Research on recombinant NSMCE1 has advanced understanding of its role in preventing pathological chromosome missegregation and fragile site instability. Defects in NSMCE1 or the SMC5/6 complex are linked to mitotic errors, premature aging phenotypes, and cancer predisposition. The protein's recombinant form enables in vitro reconstitution of complex activities, screening for potential inhibitors, and structural analysis of its interaction interfaces. Current investigations focus on how NSMCE1 coordinates with other DNA repair machinery to resolve replication stress and maintain genomic fidelity, particularly in contexts of oncogene-induced replication overload. These studies provide insights into fundamental chromosome biology and potential therapeutic strategies targeting DNA repair pathways in cancer treatment.
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