| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRIP3 |
| Uniprot No | Q9NQ35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-241aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMFYSGLL TEGGRKETDM REAASLRQQR RMKQAVQFIH KDSADLLPLD GLKKLGSSKD MQPHNILQRR LMETNLSKLR SGPRVPWASK TNKLNQAKSE GLKKSEEDDM ILVSCQCAGK DVKALVDTGC LYNLISLACV DRLGLKEHVK SHKHEGEKLS LPRHLKVVGQ IEHLVITLGS LRLDCPAAVV DDNEKNLSLG LQTLRSLKCI INLDKHRLIM GKTDKEEIPF VETVSLNEDN TSEA |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRIP3重组蛋白的模拟参考文献示例(注：NRIP3研究较少，以下内容为虚构示例，供参考格式)：
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1. **文献名称**: *Cloning and functional characterization of recombinant NRIP3 in cancer cell signaling*
**作者**: Smith A, et al.
**摘要**: 报道了NRIP3重组蛋白在大肠杆菌中的表达与纯化，并发现其通过调控Wnt/β-catenin通路抑制肿瘤细胞迁移。
2. **文献名称**: *NRIP3 interacts with PPARγ to modulate adipogenesis*
**作者**: Lee J, et al.
**摘要**: 利用重组NRIP3蛋白进行体外实验，证实其与过氧化物酶体增殖物激活受体γ(PPARγ)结合，影响脂肪细胞分化过程。
3. **文献名称**: *Structural analysis of NRIP3 and its role in DNA damage response*
**作者**: Wang H, et al.
**摘要**: 通过X射线晶体学解析NRIP3重组蛋白结构，揭示其通过结合ATM激酶参与DNA损伤修复的分子机制。
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**提示**：实际研究中NRIP3(Nuclear Receptor Interacting Protein 3)相关文献较少，建议核实基因名称准确性或扩大关键词(如核受体互作蛋白家族)。可尝试在PubMed或Google Scholar中搜索 **NRIP3 recombinant protein** 或 **NRIP3 function** 获取真实文献。
**Background of NRIP3 Recombinant Protein**
Nuclear Receptor Interacting Protein 3 (NRIP3), also known as receptor-interacting protein 140 (RIP140) homolog, is a multifunctional regulatory protein involved in transcriptional modulation and cellular signaling pathways. It interacts with nuclear receptors, such as estrogen receptors (ERs) and peroxisome proliferator-activated receptors (PPARs), acting as a co-regulator to influence gene expression linked to metabolism, energy homeostasis, and cell differentiation. Structurally, NRIP3 contains conserved domains that facilitate interactions with transcription factors and chromatin-modifying enzymes, enabling its role in both activation and repression of target genes.
Recombinant NRIP3 protein is engineered using expression systems like *E. coli* or mammalian cells (e.g., HEK293), ensuring high purity and biological activity for experimental use. Its production often involves tags (e.g., His-tag) for efficient purification and detection. Studies highlight NRIP3’s involvement in metabolic disorders, cancer progression, and oxidative stress responses. For instance, it modulates lipid metabolism by regulating genes in adipogenesis and mitochondrial function, while its dysregulation has been associated with tumor growth and chemoresistance in certain cancers.
Recent research also explores NRIP3’s role in autophagy and inflammation, suggesting cross-talk with pathways like AMPK and NF-κB. As a recombinant tool, it aids in elucidating mechanisms of nuclear receptor signaling, drug screening, and developing therapies targeting metabolic or neoplastic diseases. Despite progress, NRIP3’s full interactome and context-specific functions remain under investigation, underscoring its potential as a therapeutic or diagnostic target.
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