| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRK1 |
| Uniprot No | Q9NWW6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKTFIIG ISGVTNSGKT TLAKNLQKHL PNCSVISQDD FFKPESEIET DKNGFLQYDV LEALNMEKMM SAISCWMESA RHSVVSTDQE SAEEIPILII EGFLLFNYKP LDTIWNRSYF LTIPYEECKR RRSTRVYQPP DSPGYFDGHV WPMYLKYRQE MQDITWEVVY LDGTKSEEDL FLQVYEDLIQ ELAKQKCLQV TA |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRK1重组蛋白的3篇示例参考文献(注：文献为虚构示例，实际研究中请通过学术数据库查询真实文献)：
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1. **标题**: "Functional Characterization of Recombinant NRK1 Kinase in Cell Cycle Regulation"
**作者**: Takeda, K., et al.
**摘要**: 本研究通过在大肠杆菌系统中表达并纯化重组NRK1蛋白，揭示了其在G2/M期细胞周期调控中的作用。实验表明，NRK1通过磷酸化Cdc25磷酸酶调控CDK1活性，进而影响有丝分裂进程。
2. **标题**: "Structural Insights into NRK1 Kinase Domain and Its Substrate Specificity"
**作者**: Abe, S., et al.
**摘要**: 利用X射线晶体学解析了重组NRK1激酶结构域的3D结构，结合体外激酶实验，发现其活性位点中的Asp-312残基对底物Plk1的识别至关重要，为设计NRK1特异性抑制剂提供了结构基础。
3. **标题**: "Recombinant NRK1 Expression in Insect Cells and Its Role in DNA Damage Response"
**作者**: Smith, J.L., et al.
**摘要**: 在杆状病毒-昆虫细胞系统中高效表达功能性NRK1重组蛋白，并证明其在DNA损伤条件下通过激活ATM/ATR信号通路参与细胞凋亡调控，为研究NRK1在肿瘤治疗中的作用提供了实验工具。
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如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词(如“NRK1 recombinant protein”或“NRK1 kinase”)，并筛选近年发表的研究。
**Background of NRK1 Recombinant Protein**
NRK1 (nicotinamide riboside kinase 1) is an enzyme encoded by the *NRK1* gene, belonging to the kinase family. It plays a critical role in cellular metabolism by catalyzing the phosphorylation of nicotinamide riboside (NR), a precursor of nicotinamide adenine dinucleotide (NAD+), into nicotinamide mononucleotide (NMN). NAD+ is an essential coenzyme involved in redox reactions, energy production, DNA repair, and signaling pathways linked to aging and stress response.
Recombinant NRK1 protein is engineered through molecular cloning, where the *NRK1* gene is expressed in heterologous systems like *E. coli* or mammalian cells. This allows large-scale production of purified, functional NRK1 for research applications. The recombinant form retains enzymatic activity, enabling studies on NAD+ biosynthesis, substrate specificity, and interactions with inhibitors or activators.
NRK1 has garnered attention due to its potential therapeutic implications. Dysregulation of NAD+ metabolism is associated with aging, neurodegenerative diseases (e.g., Alzheimer’s), and metabolic disorders. By modulating NRK1 activity, researchers aim to boost NAD+ levels, which may mitigate age-related decline or metabolic dysfunction. Additionally, NRK1 is studied in cancer biology, as some tumors exploit NAD+ pathways for growth.
Structural and biochemical analyses of recombinant NRK1 have clarified its mechanism, including ATP-binding domains and catalytic residues. Its recombinant version is also used in drug screening to identify molecules targeting NAD+-dependent processes.
In summary, recombinant NRK1 serves as a vital tool for dissecting NAD+ metabolism and developing therapies for diseases linked to cellular energy imbalance and aging.
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