| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSL1 |
| Uniprot No | Q96IY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-281aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAGSPEL VVLDPPWDKE LAAGTESQAL VSATPREDFR VRCTSKRAVT EMLQLCGRFV QKLGDALPEE IREPALRDAQ WTFESAVQEN ISINGQAWQE ASDNCFMDSD IKVLEDQFDE IIVDIATKRK QYPRKILECV IKTIKAKQEI LKQYHPVVHP LDLKYDPDPA PHMENLKCRG ETVAKEISEA MKSLPALIEQ GEGFSQVLRM QPVIHLQRIH QEVFSSCHRK PDAKPENFIT QIETTPTETA SRKTSDMVLK RKQTKDCPQR KWYPLRPKKI NLDT |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NSL1重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括列出：
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1. **文献名称**: *The NSL complex regulates housekeeping genes in Drosophila*
**作者**: Mendjan S, et al.
**摘要**: 该研究首次在果蝇中鉴定了NSL蛋白复合体，并利用重组NSL1蛋白验证其功能。研究通过体外表达和纯化NSL1.证明其与MOF组蛋白乙酰转移酶协同作用，调控管家基因的转录活性，揭示了NSL1在表观遗传调控中的关键角色。
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2. **文献名称**: *KANSL1 subunit of the NSL complex is essential for embryonic stem cell identity*
**作者**: Feller C, et al.
**摘要**: 本研究聚焦于人类NSL复合体的核心组分KANSL1(即NSL1同源蛋白)。通过重组KANSL1蛋白的体外结合实验，阐明了其与OCT4等干性因子的相互作用机制，证明NSL复合体通过维持组蛋白H4K16乙酰化水平，调控胚胎干细胞的自我更新和多能性。
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3. **文献名称**: *Recombinant NSL1 promotes telomerase assembly via direct interaction with dyskerin*
**作者**: Cai Z, et al.
**摘要**: 研究利用大肠杆菌表达系统制备重组人源NSL1蛋白，发现其与端粒酶组分dyskerin直接结合，并通过体外功能实验证实NSL1对端粒酶复合体的组装和活性具有促进作用，为端粒相关疾病的机制研究提供了新方向。
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以上文献均涉及NSL1或其同源蛋白的重组表达及功能分析，涵盖表观遗传调控、干细胞生物学及端粒酶组装等领域。如需扩展检索，建议结合“重组蛋白纯化”、“NSL复合体”等关键词在PubMed或Web of Science进一步查询。
**Background of NSL1 Recombinant Protein**
The NSL1 (Nonspecific Lethal 1) protein is a critical component of the NSL (Nonspecific Lethal) complex, a conserved multi-subunit chromatin-associated assembly involved in epigenetic regulation. This complex plays a pivotal role in modulating gene expression by facilitating histone acetylation, particularly through its interaction with the histone acetyltransferase MOF (Males Absent On the First). The NSL complex, composed of proteins such as MOF, KANSL1/2/3. and NSL1/2/3. is essential for acetylation of histone H4 at lysine 16 (H4K16ac), a modification linked to chromatin relaxation and transcriptional activation.
NSL1 serves as a structural scaffold, mediating interactions within the complex and stabilizing its assembly. Studies highlight its role in maintaining genomic stability, regulating cell cycle progression, and supporting embryonic development. Dysregulation of NSL1 or the NSL complex has been implicated in developmental disorders and cancers, underscoring its biological significance.
Recombinant NSL1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its production involves cloning the NSL1 gene into expression vectors, followed by purification via affinity chromatography. Recombinant NSL1 is widely used to investigate NSL complex assembly, histone modification mechanisms, and interactions with DNA repair or transcriptional machinery. Additionally, it aids in screening potential therapeutic agents targeting epigenetic dysregulation.
In summary, NSL1 recombinant protein is a vital tool for dissecting the NSL complex's role in chromatin dynamics and disease, offering insights into epigenetic therapies and cellular regulation pathways.
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