| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTS |
| Uniprot No | P30990 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-170aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMSDSEEEMKALEADFLTNMHTSKISK AHVPSWKMTLLNVCSLVNNLNSPAEETGEVHEEELVARRKLPTALDGFSL EAMLTIYQLHKICHSRAFQHWELIQEDILDTGNDKNGKEEVIKRKIPYIL KRQLYENKPRRPYILKRDSYYY |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NTS(神经降压素)重组蛋白研究的示例参考文献(注：以下文献为示例，实际引用时请核实真实性和可用性)：
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1. **文献名称**: *"High-yield Expression and Purification of Neurotensin Recombinant Protein in E. coli"*
**作者**: Zhang, Y. et al. (2018)
**摘要**: 本研究报道了一种通过大肠杆菌表达系统高效制备NTS重组蛋白的方法，优化了诱导条件和纯化步骤，获得高纯度、具有生物活性的神经降压素，为后续功能研究奠定基础。
2. **文献名称**: *"Structural and Functional Analysis of Recombinant Neurotensin in Receptor Binding"*
**作者**: Thompson, R.L. et al. (2020)
**摘要**: 通过X射线晶体学解析了重组NTS蛋白的三维结构，并验证了其C端区域在神经降压素受体(NTSR1)结合中的关键作用，揭示了其信号转导的分子机制。
3. **文献名称**: *"Targeted Drug Delivery Using Neurotensin-Conjugated Nanoparticles Loaded with Recombinant NTS Protein"*
**作者**: Lee, S. et al. (2019)
**摘要**: 开发了一种基于重组NTS蛋白的靶向递送系统，证明其可特异性结合过表达NTSR1的癌细胞，增强化疗药物在肿瘤模型中的富集和疗效。
4. **文献名称**: *"Recombinant Neurotensin Modulates Inflammatory Responses in Neurodegenerative Disease Models"*
**作者**: Chen, X. et al. (2021)
**摘要**: 利用重组NTS蛋白处理小鼠神经炎症模型，发现其通过抑制小胶质细胞过度活化减轻脑部炎症损伤，提示其在神经退行性疾病中的潜在治疗价值。
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建议通过PubMed、Web of Science或Google Scholar搜索关键词“recombinant neurotensin protein”获取真实文献。
**Background of NTS Recombinant Protein**
NTS (Neurotensin) recombinant protein is a biologically engineered molecule derived from the neuropeptide neurotensin, which is naturally produced in the central nervous system and gastrointestinal tract. Neurotensin, first isolated in the 1970s, plays multifunctional roles as a neurotransmitter, hormone, and neuromodulator. It interacts with specific receptors (NTSR1. NTSR2. and NTSR3) to regulate processes such as pain perception, dopamine signaling, intestinal motility, and tumor growth.
The development of recombinant NTS protein leverages advances in genetic engineering and protein expression systems. By cloning the NTS gene into bacterial, yeast, or mammalian cell lines, researchers produce large quantities of purified, bioactive NTS protein with high consistency. This approach overcomes limitations of natural extraction, such as low yield and batch variability, enabling scalable production for research and therapeutic applications.
NTS recombinant protein is widely used in studying receptor-ligand interactions, signal transduction pathways, and disease mechanisms, particularly in cancer and neurological disorders. For instance, NTSR1 overexpression in certain cancers has spurred interest in NTS-based diagnostic tools or targeted therapies. Additionally, its role in modulating dopamine systems links it to conditions like schizophrenia and Parkinson’s disease, making it a valuable tool in neuroscience research.
Recent advancements in protein engineering, such as site-specific mutagenesis or fusion tags, have enhanced NTS recombinant protein’s stability, solubility, and functional specificity. These modifications support its application in structural biology, drug screening, and preclinical models.
Overall, NTS recombinant protein represents a critical reagent bridging basic research and translational medicine, offering insights into complex biological systems and therapeutic potential across diverse diseases.
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