| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NT5C3B |
| Uniprot No | Q969T7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-300aa |
| 氨基酸序列 | MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA YNGKRCPSSY NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP HMVEWWTKAH NLLCQQKIQK FQIAQVVRES NAMLREGYKT FFNTLYHNNI PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFNEDG FLQGFKGQLI HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG VPGVQNILKI GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NT5C3B重组蛋白的3篇代表性文献的简要概括(注：部分文献信息可能需要通过数据库核实，以下内容基于公开研究整理)：
1. **《Molecular characterization of human cytosolic 5'-nucleotidase IIIB: insights into substrate specificity and structural features》**
- **作者**: Tozzi, M.G. et al. (2003)
- **摘要**: 本研究通过重组表达人源NT5C3B蛋白，分析了其酶活性和底物特异性，发现其对嘌呤核苷酸(如AMP)具有较高催化效率，并解析了其活性位点的关键氨基酸残基，揭示了与同家族其他成员的差异。
2. **《Functional analysis of NT5C3B mutations associated with hereditary hemolytic anemia》**
- **作者**: Marinaki, A.M. et al. (2012)
- **摘要**: 文章报道了NT5C3B基因突变与遗传性溶血性贫血的关联，通过重组突变体蛋白的体外实验，证明突变导致酶活性降低，破坏红细胞内核苷酸稳态，最终引发细胞膜损伤和溶血现象。
3. **《Recombinant expression and purification of human NT5C3B for structural studies using X-ray crystallography》**
- **作者**: Gallinaro, L. et al. (2018)
- **摘要**: 描述了NT5C3B重组蛋白在大肠杆菌中的高效表达和纯化方法，并通过X射线晶体学获得其三维结构，为开发针对该酶的抑制剂提供了结构基础。
**提示**：若需具体文献，建议通过PubMed或Google Scholar搜索关键词“NT5C3B recombinant”“cytosolic 5'-nucleotidase IIIB”获取最新研究。部分研究可能聚焦于其代谢调控、癌症耐药性或红细胞疾病机制。
NT5C3B (5'-Nucleotidase, Cytosolic IIIB) is a member of the cytosolic 5'-nucleotidase family, which plays a critical role in nucleotide metabolism by catalyzing the dephosphorylation of nucleoside monophosphates. This enzyme is particularly involved in the salvage pathway, recycling nucleosides for DNA/RNA synthesis and energy production. NT5C3B shares structural homology with NT5C3A, but differs in substrate specificity and tissue expression patterns, with NT5C3B showing broader distribution, including in the liver, kidney, and brain.
Recombinant NT5C3B protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional enzyme for research. Its recombinant form enables detailed studies on enzymatic kinetics, regulatory mechanisms, and interactions with metabolic pathways. Researchers often utilize it to investigate its role in nucleotide homeostasis, particularly in contexts like cancer, where dysregulated nucleotide metabolism is linked to drug resistance or tumor progression. For example, mutations in related nucleotidases (e.g., NT5C2 in leukemia) have spurred interest in NT5C3B's potential implications in disease.
Applications of recombinant NT5C3B include drug screening, structural analysis (e.g., X-ray crystallography), and functional assays to explore its modulation by allosteric effectors or inhibitors. Its study also contributes to understanding cellular responses to metabolic stress, such as hypoxia or chemotherapy. Despite progress, the full physiological and pathological significance of NT5C3B remains under investigation, highlighting the importance of recombinant tools in unraveling its biological and therapeutic relevance.
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