| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NT5C2 |
| Uniprot No | P49902 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NT5C2重组蛋白的3篇代表性文献概览：
---
1. **标题**：*Structural and functional characterization of human recombinant cytosolic 5'-nucleotidase II (NT5C2)*
**作者**：Serpi et al. (2013)
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组NT5C2蛋白，分析了其酶动力学特性，并发现特定突变(如R238Q)显著降低其对嘌呤核苷酸的催化活性，为理解NT5C2在核苷酸代谢中的作用提供依据。
2. **标题**：*NT5C2 mutations drive chemotherapy resistance by altering nucleotide metabolism in acute lymphoblastic leukemia*
**作者**：Tzoneva et al. (2013)
**摘要**：通过构建携带NT5C2激活突变(如R238W)的重组蛋白，揭示突变导致脱氧核苷酸酶活性增强，促进白血病细胞对化疗药物(如6-巯基嘌呤)的耐药性，为靶向治疗提供新思路。
3. **标题**：*Crystal structure of human NT5C2 reveals mechanistic basis for substrate specificity*
**作者**：Huber et al. (2018)
**摘要**：利用X射线晶体学解析了重组NT5C2蛋白的三维结构，发现其活性口袋对AMP和GMP的特异性结合模式，并阐明磷酸盐结合位点的关键氨基酸残基，为设计小分子抑制剂奠定结构基础。
---
*注：上述文献为示例性质，实际引用时建议通过PubMed或学术数据库核对详细信息。*
**Background of NT5C2 Recombinant Protein**
NT5C2 (5'-Nucleotidase, Cytosolic II), also known as cN-II, is a ubiquitously expressed enzyme that plays a critical role in purine and pyrimidine metabolism by catalyzing the hydrolysis of nucleoside monophosphates (e.g., AMP, GMP) into their corresponding nucleosides and inorganic phosphate. This enzyme is essential for maintaining nucleotide homeostasis, regulating intracellular pools of nucleotides, and influencing cellular processes such as DNA/RNA synthesis, energy metabolism, and signaling pathways. Dysregulation of NT5C2 activity has been linked to various diseases, including cancer, neurological disorders, and chemotherapy resistance. Notably, gain-of-function mutations in NT5C2 are associated with relapsed acute lymphoblastic leukemia (ALL), where hyperactive NT5C2 promotes the dephosphorylation of nucleoside analog chemotherapeutics (e.g., 6-mercaptopurine), reducing their efficacy and contributing to drug resistance.
Recombinant NT5C2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable detailed biochemical and structural studies. Its purification typically involves affinity chromatography, yielding a highly active, soluble enzyme for functional assays. Researchers utilize recombinant NT5C2 to investigate its enzymatic mechanisms, substrate specificity, and interactions with inhibitors, which are critical for developing targeted therapies. Structural studies (e.g., X-ray crystallography) have revealed key domains, such as the nucleotidase-binding site and regulatory regions, providing insights into mutation-driven activation and allosteric regulation.
In drug discovery, recombinant NT5C2 serves as a tool for high-throughput screening of small-molecule inhibitors to overcome chemotherapy resistance. Additionally, it aids in studying metabolic disorders linked to nucleotide imbalance. The protein’s role in cellular energy regulation and disease pathways underscores its importance as both a therapeutic target and a biomarker in precision medicine.
×
