| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NT5M |
| Uniprot No | Q9NPB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-228aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGGRALRVLVDMDGVLADFEGGFLRKFRAR FPDQPFIALEDRRGFWVS EQYGRLRPGLSEKAISIWESKNFFFELEPL PGAVEAVKEMASLQNTDVFICTSPIKMFKYCPYEKYAWVEKYFGP DFL EQIVLTRDKTVVSADLLIDDRPDITGAEPTPSWEHVLFTACHNQHLQLQP PRRRLHSWADDWKAILDSKRPC |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NT5M重组蛋白的3篇参考文献(信息为模拟示例，实际文献需通过学术数据库检索获取)：
---
1. **文献名称**: _Recombinant expression and functional characterization of human NT5M in nucleotide metabolism_
**作者**: Smith J, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组人源NT5M蛋白，证实其具有水解胞嘧啶核苷酸的酶活性，并发现其活性依赖镁离子的存在，为研究核苷酸代谢调控机制提供了工具。
---
2. **文献名称**: _Structural insights into NT5M-mediated drug resistance in leukemia cells_
**作者**: Chen L, et al.
**摘要**: 利用重组NT5M蛋白进行晶体结构解析，揭示了其与抗癌药物6-巯基嘌呤的相互作用位点，阐明了NT5M过表达导致白血病细胞耐药性的分子基础。
---
3. **文献名称**: _NT5M knockout mice exhibit impaired mitochondrial function via recombinant protein complementation assay_
**作者**: Tanaka K, et al.
**摘要**: 通过重组NT5M蛋白回补实验，证明NT5M缺失小鼠线粒体功能异常与其核苷酸补救途径缺陷相关，提示NT5M在能量代谢中的关键作用。
---
建议通过PubMed或Web of Science以 **"NT5M" OR "5'-nucleotidase mitochondrial"** + **"recombinant"** 为关键词检索最新文献，重点关注其与代谢疾病、癌症耐药性及酶动力学相关研究。
**Background of NT5M Recombinant Protein**
NT5M, also known as cytosolic 5'-nucleotidase 1A (NT5C1A), is an enzyme encoded by the *NT5C1A* gene in humans. It belongs to the 5'-nucleotidase family and plays a critical role in nucleotide metabolism by catalyzing the dephosphorylation of purine and pyrimidine nucleoside monophosphates, such as AMP and IMP, into their corresponding nucleosides. This activity regulates intracellular nucleotide pools, which are essential for DNA/RNA synthesis, energy transfer, and cellular signaling. NT5M is primarily localized in the cytoplasm and is involved in maintaining nucleotide homeostasis, particularly in tissues with high metabolic demands, such as skeletal muscle, liver, and brain.
Dysregulation of NT5M has been implicated in various pathological conditions. For instance, mutations in *NT5C1A* are associated with inclusion body myopathy (IBM), a degenerative muscle disorder. Additionally, altered NT5M expression or activity may influence cellular responses to stress, such as hypoxia or chemotherapy, by modulating nucleotide availability.
Recombinant NT5M protein is produced via genetic engineering in heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functional consistency. This engineered protein retains enzymatic activity and is widely used in biochemical assays, drug discovery, and mechanistic studies to explore its role in nucleotide metabolism, muscle diseases, and cancer biology. Researchers also employ NT5M recombinant protein to screen inhibitors or activators, aiming to develop therapeutic strategies targeting nucleotide-related disorders. Its structural and functional characterization continues to provide insights into cellular energetics and disease mechanisms.
×
