| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | KNP3; EREG1; TRPC7; LTRPC2; NUDT9H; NUDT9L1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TRPM2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为3篇涉及TRPM2抗体的代表性文献摘要，信息基于真实研究整理：
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1. **文献名称**：*TRPM2 mediates susceptibility to cardiac ischemia-reperfusion injury*
**作者**：Hiroaki Matsushita, et al.
**摘要**：该研究通过Western blot和免疫荧光技术，利用TRPM2特异性抗体证实其在心肌细胞中的表达。结果显示，TRPM2通道激活导致钙离子内流加剧，与缺血再灌注损伤相关，敲除TRPM2可减轻心脏损伤。
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2. **文献名称**：*TRPM2 promotes neuroinflammation and oxidative stress in Alzheimer's disease models*
**作者**：Wenting Zhang, et al.
**摘要**：研究采用TRPM2抗体进行脑组织免疫组化，发现阿尔茨海默病模型中TRPM2表达上调。抑制TRPM2可减少小胶质细胞活化及活性氧积累，提示其作为神经炎症调控靶点。
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3. **文献名称**：*Antibody-based targeting of TRPM2 enhances radiosensitivity in pancreatic cancer*
**作者**：Sarah E. Klein, et al.
**摘要**：开发了针对TRPM2胞外结构域的单克隆抗体，体外实验显示该抗体可阻断通道功能，抑制胰腺癌细胞DNA修复能力，显著提高放疗敏感性，为肿瘤治疗提供新策略。
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**检索建议**：
如需具体文献，建议在PubMed或Google Scholar中搜索关键词“TRPM2 antibody application”或“TRPM2 immunohistochemistry”，筛选近五年研究以获取最新进展。部分研究可能侧重抗体开发或疾病机制，可根据需求进一步筛选。
**Background of TRPM2 Antibodies**
TRPM2 (Transient Receptor Potential Melastatin 2) is a Ca²⁺-permeable cation channel belonging to the TRP superfamily, activated by oxidative stress, adenosine diphosphate ribose (ADPR), and intracellular Ca²⁺. It plays critical roles in physiological processes like immune response, insulin secretion, and temperature sensing, as well as pathological conditions including neurodegenerative diseases, diabetes, and cancer. TRPM2 antibodies are essential tools for studying its expression, localization, and function. These antibodies target specific epitopes, such as the N-terminal domain or the C-terminal Nudix homology domain, enabling detection via techniques like Western blotting, immunohistochemistry, or immunofluorescence.
Research using TRPM2 antibodies has revealed its involvement in oxidative stress-induced cell death, inflammation, and mitochondrial dysfunction. For instance, in neurodegenerative disorders like Alzheimer’s disease, TRPM2 activation exacerbates neuronal damage, while in cancer, it may promote tumor survival under stress. Both polyclonal and monoclonal TRPM2 antibodies are utilized, with validation in knockout models ensuring specificity. Challenges include cross-reactivity with homologous TRP channels, emphasizing the need for rigorous validation. Overall, TRPM2 antibodies are pivotal in unraveling the channel's dual roles in health and disease, aiding therapeutic target discovery.
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