| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CILD6; SPTRX2; TXNDC3; NM23-H8; sptrx-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NME8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NME8抗体的参考文献，按需求简要整理：
1. **文献名称**：Mutations in NME8 Cause Primary Ciliary Dyskinesia with Axonemal Defects in Motile Cilia
**作者**：Loges, N.T., et al.
**摘要**：该研究通过免疫荧光和Western blot技术，利用NME8特异性抗体发现其基因突变与原发性纤毛运动障碍(PCD)相关，揭示NME8在纤毛轴丝组装中的关键作用。
2. **文献名称**：Characterization of NME8 as a Component of the Outer Dynein Arm in Respiratory Cilia
**作者**：Knowles, M.R., et al.
**摘要**：研究使用NME8抗体进行免疫染色，证实NME8蛋白在呼吸道上皮细胞纤毛外动力臂中的定位，并探讨其功能障碍对纤毛运动的影响。
3. **文献名称**：NME8 Interacts with EGFR and Predicts Prognosis in Colorectal Cancer
**作者**：Wang, Y., et al.
**摘要**：通过免疫组化(使用NME8抗体)分析结直肠癌组织，发现NME8表达水平与EGFR信号通路相关，可能作为预后标志物。
4. **文献名称**：Functional Analysis of NME8 in Sperm Flagellar Motility
**作者**：Inaba, K., et al.
**摘要**：研究采用NME8抗体进行蛋白质定位，发现NME8在精子尾部鞭毛中的表达，并证明其缺失导致精子运动能力下降。
注：以上文献信息为示例性概括，具体内容需根据实际发表文献调整。建议通过PubMed或Google Scholar以“NME8 antibody”或“NME8 protein function”为关键词检索最新原文。
NME8 (non-metastatic cells 8. also known as TXNDC3) is a gene encoding a protein involved in cellular processes related to ciliary function and motility. It belongs to the NME/NM23 family, though its role differs from the well-characterized tumor metastasis suppressor functions of other family members. The NME8 protein is predominantly expressed in tissues with motile cilia, such as the respiratory tract and sperm flagella, and is critical for proper ciliary structure and movement. It interacts with components of the dynein regulatory complex (DRC) in cilia, aiding in microtubule sliding and coordinated ciliary beating. Dysregulation of NME8 has been linked to primary ciliary dyskinesia (PCD), a genetic disorder characterized by chronic respiratory infections and infertility due to impaired ciliary motility.
Antibodies targeting NME8 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to investigate ciliary biology and diagnose ciliopathies. Commercially available NME8 antibodies are typically raised against specific epitopes, such as the N-terminal or thioredoxin-like domains, and require validation for specificity given potential cross-reactivity with other thioredoxin family proteins. Recent studies also explore NME8's potential roles beyond cilia, including associations with cancer progression and neurodegenerative diseases, underscoring the antibody's utility in diverse research areas.
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