| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUBP2 |
| Uniprot No | Q9Y5Y2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-271aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEAAAEP GNLAGVRHII LVLSGKGGVG KSTISTELAL ALRHAGKKVG ILDVDLCGPS IPRMLGAQGR AVHQCDRGWA PVFLDREQSI SLMSVGFLLE KPDEAVVWRG PKKNALIKQF VSDVAWGELD YLVVDTPPGT SDEHMATIEA LRPYQPLGAL VVTTPQAVSV GDVRRELTFC RKTGLRVMGI VENMSGFTCP HCTECTSVFS RGGGEELAQL AGVPFLGSVP LDPALMRTLE EGHDFIQEFP GSPAFAALTS IAQKILDATP ACLP |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUBP2重组蛋白的示例参考文献(内容为虚构示例，仅供参考)：
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1. **文献名称**：NUBP2重组蛋白与MMS19相互作用在铁硫簇组装中的功能研究
**作者**：Zhang Y. et al.
**摘要**：本研究通过体外重组表达纯化NUBP2蛋白，发现其与MMS19形成复合物，参与细胞内铁硫簇的组装过程，并验证了该复合物对DNA修复酶活性的调控作用。
2. **文献名称**：NUBP2重组蛋白在结直肠癌细胞增殖中的调控机制
**作者**：Wang L. et al.
**摘要**：利用重组NUBP2蛋白进行功能实验，证明其过表达抑制结直肠癌细胞增殖和迁移，可能与Wnt/β-catenin信号通路的下调相关。
3. **文献名称**：NUBP2重组蛋白的晶体结构解析及ATP结合特性
**作者**：Lee S. et al.
**摘要**：通过X射线晶体学首次解析了NUBP2重组蛋白的三维结构，揭示了其核苷酸结合域的关键氨基酸残基，为靶向药物设计提供结构基础。
4. **文献名称**：NUBP2重组蛋白在氧化应激中的抗氧化功能研究
**作者**：Patel R. et al.
**摘要**：实验表明，重组NUBP2蛋白通过调节细胞内活性氧(ROS)水平，减轻氧化应激诱导的细胞损伤，提示其在神经退行性疾病中的潜在应用价值。
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**注**：以上文献信息为模拟示例，实际研究需通过PubMed、Google Scholar等平台检索真实文献。
NUBP2 (Nucleotide-Binding Protein 2), also known as cytosolic Fe-S cluster assembly factor 2. is a conserved eukaryotic protein belonging to the NUBP/MRP subfamily of ATP-binding proteins. It plays a critical role in cellular iron-sulfur (Fe-S) cluster biogenesis, a fundamental process essential for mitochondrial function, DNA repair, and enzyme activities. NUBP2 typically forms a heterotetrameric complex with its homolog NUBP1. functioning as a cytosolic Fe-S cluster assembly (CIA) machinery component. This complex facilitates the transfer of Fe-S clusters to target apoproteins, including key enzymes involved in nucleotide metabolism and redox regulation.
Recombinant NUBP2 protein is engineered through molecular cloning, often expressed in bacterial (e.g., E. coli) or mammalian systems for biochemical studies. Its purified form enables structural analysis, interaction assays, and functional characterization. Researchers utilize recombinant NUBP2 to investigate its ATPase activity, Fe-S cluster binding properties, and dynamic interplay with partner proteins like NUBP1 and CIA targeting factors. Structural studies reveal conserved nucleotide-binding domains and Fe-S coordination sites critical for its chaperone function.
Dysregulation of NUBP2 has been linked to human diseases, including cancers and neurodegenerative disorders, where Fe-S cluster homeostasis is compromised. Its recombinant form serves as a tool for drug screening and mechanistic studies targeting Fe-S-related pathologies. Current research also explores its potential as a biomarker or therapeutic target in malignancies characterized by altered iron metabolism or genomic instability.
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