| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUCB2 |
| Uniprot No | P80303 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-106aa |
| 氨基酸序列 | MKHHHHHHASVPIDIDKTKVQNIHPVESAKIEPPDTGLYYDEYLKQVIDV LETDKHFREKLQKADIEEIKSGRLSKELDLVSHHVRTKLDEL |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUCB2重组蛋白的模拟参考文献示例(部分信息为合理推测，建议通过学术数据库核实具体文献)：
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1. **标题**：*"Recombinant Expression and Functional Characterization of Human NUCB2 in a Bacterial System"*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达了带有His标签的重组人源NUCB2蛋白，通过亲和层析纯化后，利用圆二色谱和体外结合实验证实其具有与天然蛋白相似的二级结构及钙离子结合活性，为后续功能研究提供基础。
2. **标题**：*"Recombinant Nesfatin-1/NUCB2 Suppresses Food Intake via mTOR Signaling in Hypothalamic Neurons"*
**作者**：Zhang Y, et al.
**摘要**：通过哺乳动物细胞系统表达重组NUCB2蛋白并酶解获得活性nesfatin-1片段。体外实验表明，重组nesfatin-1可通过激活下丘脑神经元mTOR通路抑制食欲，提示其在代谢疾病中的潜在应用。
3. **标题**：*"Crystal Structure Analysis of Recombinant NUCB2 Reveals Key Domains for Cellular Localization"*
**作者**：Tanaka R, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度NUCB2重组蛋白，通过X射线晶体学解析其三维结构，发现N端结构域对核定位至关重要，为理解其生理功能提供结构基础。
4. **标题**：*"Therapeutic Effects of Recombinant NUCB2 in a Rodent Model of Metabolic Syndrome"*
**作者**：Gupta S, et al.
**摘要**：在代谢综合征大鼠模型中，注射重组NUCB2蛋白显著改善胰岛素抵抗和肝脏脂质沉积，其机制可能与调控脂肪因子分泌相关。
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**注意**：以上文献为模拟示例，实际研究需通过PubMed、Web of Science等平台以关键词“NUCB2 recombinant protein”“nesfatin-1 expression”检索。真实文献可能涉及具体疾病机制、蛋白工程优化或临床前研究。
NUCB2 (nucleobindin-2) is a multifunctional protein encoded by the NUCB2 gene, initially identified for its role in regulating appetite and energy homeostasis. It serves as the precursor for nesfatin-1. a bioactive peptide implicated in hypothalamic signaling pathways that control food intake and body weight. Structurally, NUCB2 is a large, conserved protein (~420 amino acids in humans) containing multiple functional domains, including a signal peptide, conserved nucleobindin (NUC) domains, and calcium-binding EF-hand motifs. These features enable its involvement in diverse cellular processes, such as calcium homeostasis, intracellular signaling, and protein-protein interactions.
Recombinant NUCB2 protein is engineered using expression systems like E. coli or mammalian cell lines to produce purified, functional forms of the protein for research and therapeutic applications. Its recombinant form retains critical biological activities, including the ability to generate nesfatin-1 through post-translational processing. Studies highlight NUCB2’s broader physiological roles beyond appetite regulation, including modulation of glucose metabolism, inflammatory responses, stress adaptation, and reproductive functions. Dysregulation of NUCB2/nesfatin-1 has been linked to metabolic disorders (e.g., obesity, diabetes), cardiovascular diseases, and certain cancers, making it a biomarker of interest.
Researchers utilize recombinant NUCB2 to dissect molecular mechanisms in vitro and in vivo, develop diagnostic assays, and explore therapeutic strategies. Challenges in its application include optimizing stability and bioavailability due to its large size and complex structure. Ongoing studies focus on elucidating receptor interactions and signaling pathways to harness its potential for treating metabolic syndromes or neuroendocrine disorders.
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