| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDCD2 |
| Uniprot No | Q8WVJ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-157aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSAPFEE RSGVVPCGTP WGQWYQTLEE VFIEVQVPPG TRAQDIQCGL QSRHVALSVG GREILKGKLF DSTIADEGTW TLEDRKMVRI VLTKTKRDAA NCWTSLLESE YAADPWVQDQ MQRKLTLERF QKENPGFDFS GAEISGNYTK GGPDFSNLEK |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDCD2重组蛋白的模拟参考文献示例(请注意，这些文献为虚构示例，仅供参考格式)：
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1. **标题**: *Cloning and Functional Characterization of Recombinant NUDCD2 in Cancer Cell Proliferation*
**作者**: Zhang L, et al.
**摘要**: 本研究通过在大肠杆菌中表达并纯化NUDCD2重组蛋白，揭示了其在白血病细胞系中通过调控Wnt/β-catenin信号通路促进细胞增殖的机制。
2. **标题**: *Structural Analysis of NUDCD2 Recombinant Protein Using X-ray Crystallography*
**作者**: Smith J, et al.
**摘要**: 作者解析了NUDCD2重组蛋白的晶体结构(分辨率为2.8Å)，发现其N端结构域对结合微管相关蛋白至关重要，为靶向药物设计提供依据。
3. **标题**: *NUDCD2 Recombinant Protein Interacts with HSP90: Implications for Chaperone-Mediated Cancer Metastasis*
**作者**: Wang Y, et al.
**摘要**: 通过体外Pull-down实验和质谱分析，证实重组NUDCD2与HSP90形成复合物，可能通过稳定致癌蛋白增强乳腺癌细胞的转移能力。
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**说明**：实际研究中，建议通过PubMed或Web of Science以关键词“NUDCD2 recombinant”或“NUDCD2 expression”检索真实文献。若研究领域较新，可扩展至相关通路(如细胞周期调控)或疾病(如肿瘤)进行关联搜索。
**Background of NUDCD2 Recombinant Protein**
NUDCD2 (NudC domain-containing protein 2) is a conserved eukaryotic protein belonging to the NudC family, initially identified for its role in nuclear migration and cell division. It shares structural homology with NudC, a critical regulator of dynein-mediated microtubule dynamics and mitotic spindle positioning. NUDCD2 is implicated in diverse cellular processes, including cell cycle regulation, cytoskeletal organization, and intracellular trafficking. Studies suggest its involvement in TGF-β and Wnt signaling pathways, influencing cell proliferation, differentiation, and apoptosis. Dysregulation of NUDCD2 has been linked to cancer progression, with overexpression observed in malignancies like colorectal, lung, and hepatocellular carcinoma, correlating with poor prognosis.
The recombinant NUDCD2 protein is engineered via molecular cloning, typically expressed in *E. coli* or mammalian systems, followed by purification using affinity chromatography (e.g., His-tag). This bioengineered tool retains the functional domains of native NUDCD2. enabling *in vitro* studies to dissect its molecular interactions, structural features, and downstream effectors. Researchers utilize it to investigate NUDCD2’s role in tumorigenesis, metastasis, and chemoresistance, as well as its potential as a therapeutic target or diagnostic biomarker. Additionally, it serves as an antigen for antibody production, aiding in immunohistochemistry, ELISA, and Western blot analyses.
Current research focuses on elucidating NUDCD2’s mechanistic contributions to disease pathways, offering insights for targeted therapies and precision medicine. Its recombinant form remains pivotal in bridging molecular biology with translational oncology.
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