| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT10 |
| Uniprot No | Q8NFP7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-164aa |
| 氨基酸序列 | MKCKPNQTRTYDPEGFKKRAACLCFRSEREDEVLLVSSSRYPDRWIVPGG GMEPEEEPGGAAVREVYEEAGVKGKLGRLLGVFEQNQDPKHRTYVYVLTV TELLEDWEDSVSIGRKREWFKVEDAIKVLQCHKPVHAEYLEKLKLGGSPT NGNSMAPSSPDSDPLEHHHHHH |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDT10重组蛋白的假设性参考文献示例(请注意，这些文献为虚构，仅用于展示格式，实际研究需通过学术数据库查询)：
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1. **文献名称**：*Recombinant Expression and Biochemical Characterization of Human NUDT10 Hydrolase*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组NUDT10蛋白，证实其具有水解ADP-核糖(ADP-ribose)的活性，并分析了其最适pH和金属离子依赖性，揭示了其在核苷酸代谢中的潜在作用。
2. **文献名称**：*Structural Insights into NUDT10 Substrate Specificity via X-ray Crystallography*
**作者**：Lee H, et al.
**摘要**：通过解析重组NUDT10蛋白的晶体结构，发现其活性位点对二磷酸核苷类底物具有独特结合模式，为设计选择性抑制剂提供了结构基础。
3. **文献名称**：*Role of NUDT10 in Oxidative Stress Response: Evidence from Recombinant Protein Assays*
**作者**：Zhang Y, et al.
**摘要**：利用重组NUDT10进行体外实验，证明其通过水解氧化损伤产生的代谢产物(如8-oxo-dGTP)减轻DNA氧化损伤，提示其在细胞抗氧化机制中的关键功能。
4. **文献名称**：*Comparative Analysis of Nudix Family Enzymes: Substrate Profiling of Recombinant NUDT10 and NUDT15*
**作者**：Brown K, et al.
**摘要**：比较NUDT10与NUDT15重组蛋白的底物特异性，发现NUDT10对ADP-核糖的催化效率显著高于其他底物，突显其在特定代谢通路中的专一性。
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**建议**：实际文献可通过PubMed、Google Scholar等平台，以关键词“NUDT10 recombinant”“NUDT10 substrate specificity”或“NUDT10 structure”检索。关注涉及酶动力学、结构生物学或疾病关联的研究。
**Background of NUDT10 Recombinant Protein**
NUDT10. a member of the Nudix hydrolase superfamily, is a conserved enzyme implicated in nucleotide metabolism and cellular signaling. This protein, also known as diadenosine hexaphosphate hydrolase 3 (DIPP3), primarily hydrolyzes dinucleoside polyphosphates (e.g., Ap₆A) and nucleotide-derived metabolites, regulating their intracellular concentrations. Its enzymatic activity relies on a conserved Nudix motif (GX₅EX₇REUXEEXGU), which facilitates metal-dependent hydrolysis, often requiring Mg²⁺ or Mn²⁺ as cofactors.
Structurally, recombinant NUDT10 is typically expressed in *E. coli* or mammalian systems, enabling studies on its substrate specificity and catalytic mechanisms. Research highlights its role in modulating signaling pathways linked to purinergic receptors, which influence processes like apoptosis, inflammation, and cell proliferation. Dysregulation of NUDT10 has been associated with pathologies such as cancer and neurodegenerative disorders, though its exact mechanistic contributions remain under investigation.
Recent studies focus on its potential as a therapeutic target, particularly in cancers with altered nucleotide metabolism. Recombinant NUDT10 serves as a tool for high-throughput screening of inhibitors or for structural analysis (e.g., X-ray crystallography) to elucidate substrate-binding sites. Its interplay with inositol pyrophosphates (e.g., IP₇) further links it to phosphate homeostasis and stress response pathways.
Despite progress, questions persist regarding its tissue-specific functions and regulatory networks. Advances in recombinant protein technology continue to drive functional studies, aiming to clarify its physiological and pathological roles, ultimately informing drug development strategies.
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