Rabbit Polyclonal VPS25 Antibody

The VPS25 antibody is a crucial tool in studying the endosomal sorting complex required for transport (ESCRT) system, specifically the ESCRT-II subunit. VPS25 (vacuolar protein sorting 25 homolog) is a conserved component of the ESCRT-II complex, which plays a vital role in membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, viral budding, and cellular cargo sorting. Researchers use VPS25 antibodies to investigate its molecular interactions, localization, and regulatory mechanisms in these pathways. These antibodies are commonly applied in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect VPS25 expression levels or track its dynamic behavior during cellular processes. Dysregulation of VPS25 has been linked to various diseases, including cancer and neurodegenerative disorders, making its study clinically relevant. For instance, VPS25 depletion can impair ESCRT-II function, leading to defective abscission during cell division or disrupted viral particle release. The antibody's specificity is typically validated using knockout cell lines or siRNA-mediated silencing to confirm signal loss. Commercial VPS25 antibodies are often raised in rabbits or mice against peptide antigens derived from conserved regions of the human protein, ensuring cross-reactivity with homologs in model organisms like mice or zebrafish. Proper controls and validation remain essential due to potential cross-reactivity with unrelated proteins in certain experimental conditions.