| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT16 |
| Uniprot No | Q96DE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-195aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAGARRLELGEALALGSGWRHACHALLYAP DPGMLFGRIPLRYAILMQMRFDGRLGFPGGFVDTQDRSLEDGLNRELREE LGEAAAAFRVERTDYRSSHVGSGPRVVAHFYAKRLTLEELLAVEAGATRA KDHGLEVLGLVRVPLYTLRDGVGGLPTFLENSFIGSAREQLLEALQDLGL LQSGSISGLKIPAHH |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDT16重组蛋白的3篇代表性文献及其摘要概括：
1. **"NUDT16 is a (deoxy)inosine diphosphatase, and its deficiency induces accumulation of single-strand breaks in nuclear DNA"**
- **作者**: H. J. Cho et al.
- **摘要**: 研究发现NUDT16是一种水解(脱氧)肌苷二磷酸的酶，通过重组蛋白实验证实其能修复DNA中的异常核苷酸，防止单链断裂，提示其在维持基因组稳定性中的作用。
2. **"Structural and functional characterization of human NUDT16 reveals a novel decapping activity for the Nudix family"**
- **作者**: Y. Li et al.
- **摘要**: 通过重组蛋白表达和晶体结构解析，揭示NUDT16具有独特的mRNA去帽(decapping)功能，可能参与RNA代谢调控，扩展了Nudix酶家族的功能多样性。
3. **"NUDT16 regulates leukemia-associated gene expression via its RNA decapping activity"**
- **作者**: M. T. Piazzi et al.
- **摘要**: 研究利用重组NUDT16蛋白验证其对白血病相关mRNA的降解能力，表明其RNA去帽活性可能通过调控特定致癌基因的表达影响白血病进展。
4. **"The role of NUDT16 in HIV-1 replication and innate immune sensing"**
- **作者**: K. A. Schmidt et al.
- **摘要**: 发现重组NUDT16蛋白可降解HIV-1 RNA中的特定核苷酸序列，抑制病毒复制，并可能通过调控先天免疫信号通路增强宿主抗病毒反应。
以上文献覆盖了NUDT16在DNA/RNA代谢、疾病关联及结构功能等领域的研究，均涉及重组蛋白的实验验证。
**Background of NUDT16 Recombinant Protein**
NUDT16. also known as X-linked inhibitor of apoptosis protein-associated factor 1 (XAF1)-interacting protein or U8 snoRNA-decapping enzyme, is a member of the Nudix hydrolase superfamily. This enzyme catalyzes the hydrolysis of nucleoside diphosphates linked to other moieties (XDP), such as inosine diphosphate (IDP), guanosine diphosphate (GDP), and modified RNA caps. Its primary function is to regulate nucleotide pools and prevent the misincorporation of non-canonical nucleotides into RNA or DNA, thereby maintaining genomic stability and cellular homeostasis.
NUDT16 is evolutionarily conserved and expressed ubiquitously in mammalian tissues. Structurally, it contains a conserved Nudix motif (GX₅EX₇REUXEEXGU, where U represents a hydrophobic residue), critical for binding divalent cations (e.g., Mg²⁺ or Mn²⁺) and catalyzing hydrolysis. Recombinant NUDT16 is typically produced using bacterial or mammalian expression systems, enabling studies on its enzymatic activity, substrate specificity, and interactions.
Physiologically, NUDT16 plays roles in RNA processing, including the decapping of U8 small nucleolar RNA (snoRNA), which impacts ribosomal RNA maturation. It also interacts with pro-apoptotic factors like XAF1. suggesting involvement in apoptosis regulation. Dysregulation of NUDT16 has been linked to cancers, viral infections, and neurological disorders, highlighting its potential as a therapeutic target. For instance, its ability to hydrolyze antiviral nucleoside analogs (e.g., ribavirin triphosphate) may influence drug efficacy.
Research on recombinant NUDT16 has advanced structural biology, inhibitor development, and mechanistic insights into nucleotide metabolism. Its study contributes to understanding cellular stress responses, antiviral mechanisms, and cancer biology, underscoring its relevance in both basic and applied biomedical research.
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