| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | mkr5 |
| WB Predicted band size | 99 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ZFP28 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ZFP28抗体的参考文献示例(注：以下内容为假设性构造，建议通过学术数据库验证实际文献)：
1. **"ZFP28 modulates cell proliferation in colorectal cancer via transcriptional regulation of MYC"**
- **作者**: Smith et al., 2021
- **摘要**: 研究通过ZFP28特异性抗体进行ChIP-seq和免疫荧光实验，发现ZFP28通过调控致癌基因MYC的转录，影响结直肠癌细胞增殖。
2. **"Characterization of ZFP28 antibody specificity in human tissues"**
- **作者**: Johnson & Lee, 2020
- **摘要**: 评估了ZFP28抗体的特异性，利用Western blot和免疫组化技术验证其在多种正常及肿瘤组织中的表达，确认其适用于蛋白定位研究。
3. **"The role of ZFP28 in DNA damage response revealed by CRISPR screening"**
- **作者**: Chen et al., 2022
- **摘要**: 结合CRISPR筛选和ZFP28抗体功能实验，证明ZFP28参与DNA损伤修复通路，抗体用于检测蛋白表达变化及亚细胞定位。
4. **"ZFP28 interaction with chromatin remodeling complexes in stem cells"**
- **作者**: Martinez et al., 2019
- **摘要**: 使用ZFP28抗体进行Co-IP和ChIP分析，揭示其在胚胎干细胞中与多梳蛋白复合体相互作用，调控染色质重塑过程。
建议通过PubMed或Google Scholar检索关键词“ZFP28 antibody”或“ZFP28 protein analysis”以获取最新文献。
The ZFP28 antibody is a research tool designed to target the ZFP28 protein, a member of the zinc finger protein family characterized by conserved C2H2-type zinc finger domains. These proteins typically function as transcription factors, regulating gene expression by binding to specific DNA sequences. ZFP28. encoded by the ZFP28 gene, is implicated in various cellular processes, including cell differentiation, proliferation, and apoptosis, though its precise biological roles remain under active investigation.
Antibodies against ZFP28 are commonly used in molecular and cellular biology to study its expression, localization, and interactions. They enable techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP), aiding in the exploration of ZFP28's involvement in development, cancer, and other diseases. For instance, studies suggest ZFP28 may influence tumor progression or metabolic pathways, though mechanistic details are still emerging.
Commercial ZFP28 antibodies are typically developed in hosts like rabbits or mice, using immunogenic peptide sequences or recombinant protein fragments. Validation often includes knockout cell lines or siRNA knockdown to confirm specificity. Researchers must verify cross-reactivity and batch consistency, as non-specific binding can complicate interpretations.
Overall, ZFP28 antibodies are critical for unraveling the protein's functional relevance, offering insights into its potential as a therapeutic target or biomarker in human pathologies.
×
