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产品描述：猪活化蛋白C(APC)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APC含量。APC（腺瘤性息肉病基因）是一种肿瘤抑制基因，其突变与结直肠癌等癌症的发生密切相关。研究机制显示，APC基因突变会导致β-catenin蛋白积累，进而激活下游原癌基因，促进肿瘤形成。通过研究APC基因及其相关信号通路，有助于开发新的癌症治疗策略。试剂盒检测范围为40 pg/mL-8000 pg/mL，该产品适用于科研领域，如猪凝血机制研究、炎症相关疾病模型（如败血症或血栓性疾病）的建立与评估，以及抗凝药物或生物制剂开发过程中药效动力学研究。其兼容多种样本类型的特点，可支持不同实验设计需求，例如通过组织匀浆分析特定器官（如肝脏或血管内皮）中APC的局部表达水平，为深入探究APC在病理生理过程中的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：APC
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：40 pg/mL-8000 pg/mL
灵敏度：20 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of pig APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 97 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.4372.4582.448 401.4621.4821.472 1601.0051.0181.012 6400.6080.6170.613 20000.3570.3420.350 80000.2000.2040.202 | ||||||
货期：3-5 working days
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