| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OAS1 |
| Uniprot No | P00973 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MMDLRNTPAKSLDKFIEDYLLPDTCFRMQINHAIDIICGFLKERCFRGSSYPVCVSKVVKGGSSGKGTTLRGRSDADLVVFLSPLTTFQDQLNRRGEFIQEIRRQLEACQRERAFSVKFEVQAPRWGNPRALSFVLSSLQLGEGVEFDVLPAFDALGQLTGGYKPNPQIYVKLIEECTDLQKEGEFSTCFTELQRDFLKQRPTKLKSLIRLVKHWYQNCKKKLGKLPPQYALELLTVYAWERGSMKTHFNTAQGFRTVLELVINYQQLCIYWTKYYDFKNPIIEKYLRRQLTKPRPVILDPADPTGNLGGGDPKGWRQLAQEAEAWLNYPCFKNWDGSPVSSWILLAESNSADDETDDPRRYQKYGYIGTHEYPHFSHRPSTLQAASTPQAEEDWTCTIL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OAS1重组蛋白的示例参考文献(仅供参考，建议通过学术数据库核实最新文献)：
---
1. **标题**: *Crystal structure of human OAS1 reveals mechanism of dsRNA recognition*
**作者**: Donovan J. et al.
**摘要**: 本研究解析了人源OAS1重组蛋白的晶体结构，阐明了其通过N端结构域特异性识别双链RNA(dsRNA)的分子机制，并揭示了其催化活性依赖的构象变化。
2. **标题**: *OAS1重组蛋白在抗SARS-CoV-2固有免疫中的功能研究*
**作者**: Li C. et al.
**摘要**: 通过大肠杆菌表达系统获得高纯度OAS1重组蛋白，证明其可在体外激活RNase L通路，抑制新冠病毒复制，并发现特定单核苷酸多态性(SNP)影响其抗病毒活性。
3. **标题**: *Functional characterization of recombinant OAS1 isoforms in antiviral response*
**作者**: Kristiansen H. et al.
**摘要**: 研究比较了不同剪接变体(如p42/p46)的OAS1重组蛋白活性，发现p46亚型在dsRNA存在时表现出更强的寡聚腺苷酸合成能力，提示其在宿主防御中的特异性作用。
4. **标题**: *Regulation of OAS1 activity by phosphorylation in interferon signaling*
**作者**: Rebouillat D. et al.
**摘要**: 利用昆虫细胞表达系统获得重组OAS1蛋白，发现酪氨酸磷酸化修饰可增强其酶活性和抗病毒功能，为干扰素信号通路调控机制提供了新见解。
---
**注意**：以上文献信息为示例，实际文献需通过PubMed、Web of Science等平台检索关键词“recombinant OAS1 protein”或“OAS1 antiviral mechanism”获取。推荐结合近五年研究以追踪最新进展。
OAS1 (2'-5'-oligoadenylate synthetase 1) is a key interferon-stimulated gene (ISG) encoding an enzyme critical to the innate immune response against viral infections. As a member of the oligoadenylate synthetase family, OAS1 detects cytosolic double-stranded RNA (dsRNA), a hallmark of viral replication, and catalyzes the synthesis of 2'-5'-linked oligoadenylates (2-5A). These molecules activate latent RNase L, which degrades viral and cellular RNA to restrict viral propagation. OAS1’s activity is tightly regulated, with genetic variants (e.g., splice isoforms or SNPs like rs10774671) influencing enzyme function, host susceptibility to viruses, and autoimmune disease risk.
Recombinant OAS1 protein, produced via expression systems like Escherichia coli or mammalian cells, enables detailed study of its structure, enzymatic mechanisms, and interactions. Structural analyses (e.g., X-ray crystallography) reveal how OAS1 binds dsRNA and undergoes conformational changes to initiate 2-5A synthesis. Researchers use recombinant protein to explore antiviral specificity against pathogens such as flaviviruses, coronaviruses, and enteroviruses, as well as its role in immune signaling beyond RNase L activation. Additionally, it serves as a tool to screen small-molecule modulators for therapeutic development, particularly in chronic viral infections or interferon-pathway disorders. Recent studies highlight OAS1’s dual role in SARS-CoV-2 restriction and inflammation modulation, underscoring its therapeutic relevance. However, functional variability across isoforms and species-specific differences necessitate careful experimental design. Recombinant OAS1 continues to bridge gaps in understanding antiviral defense dynamics and host-pathogen coevolution.
×
