| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUTF2 |
| Uniprot No | P61970 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-127aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGDKPIWEQIGSSFIQHYYQLFDNDRTQLG AIYIDASCLTWEGQQFQGKAAIVEKLSSLPFQKIQHSITAQDHQPTPDSC IISMVVGQLKADEDPIMGFHQMFLLKNINDAWVCTNDMFRLALHNFG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NUTF2重组蛋白的经典文献摘要概括：
1. **文献名称**: "Purification and characterization of recombinant human NUTF2: a key regulator of nuclear transport"
**作者**: Moore MS et al.
**摘要**: 报道了人源NUTF2重组蛋白在大肠杆菌中的高效表达及纯化方法，验证其与Ran GTPase的相互作用，阐明其在核质运输中调控核孔复合体功能的分子机制。
2. **文献名称**: "Crystal structure of the Ran-NUTF2 complex"
**作者**: Stewart M et al.
**摘要**: 通过X射线晶体学解析了重组NUTF2蛋白与Ran GTP酶的复合物三维结构，揭示NUTF2通过特定结构域识别Ran-GDP的分子机制，为核转运机制提供结构生物学证据。
3. **文献名称**: "Functional analysis of NUTF2 mutations in nuclear import deficiency"
**作者**: Yokoyama N et al.
**摘要**: 利用重组突变体NUTF2蛋白进行功能研究，发现C端结构域的氨基酸突变会破坏其与核孔蛋白的结合能力，导致细胞内蛋白核输入功能异常。
注：上述文献为基于领域知识的模拟概括，实际引用建议通过PubMed(PMID检索)或Web of Science平台验证具体文献信息。
**Background of NUTF2 Recombinant Protein**
NUTF2 (Nuclear Transport Factor 2) is a small, evolutionarily conserved protein critical for nucleocytoplasmic transport, a process essential for cellular function. It plays a pivotal role in the Ran GTPase cycle, which regulates the directional movement of macromolecules across the nuclear pore complex (NPC). Specifically, NUTF2 facilitates the recycling of RanGDP from the cytoplasm to the nucleus by binding to it and interacting with nucleoporins, components of the NPC. This activity ensures the maintenance of Ran’s concentration gradient, a prerequisite for proper nuclear import/export.
The recombinant form of NUTF2 is engineered using biotechnological platforms, such as *E. coli* or mammalian expression systems, to produce high-purity, functional protein for research and therapeutic applications. Recombinant NUTF2 retains its native structure and biochemical properties, enabling studies on its interaction with Ran, nucleoporins, and other transport factors. Its production often involves affinity tagging (e.g., His-tag) for simplified purification and detection.
Research on recombinant NUTF2 has advanced understanding of nuclear transport mechanisms, including dysregulation linked to diseases like cancer and viral infections. For instance, certain viruses exploit host transport machinery, making NUTF2 a potential target for antiviral strategies. Additionally, structural studies using recombinant NUTF2 have elucidated its binding interfaces and conformational dynamics, aiding drug design.
Beyond basic science, recombinant NUTF2 serves as a tool in synthetic biology for engineering artificial nuclear transport systems. Its role in maintaining cellular homeostasis underscores its biomedical relevance, driving continued interest in its functional characterization and applications.
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