| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT21 |
| Uniprot No | O43809 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSVVPPNRSQTGWPRGVTQFGNKYIQQTKP LTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTV EGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILG RQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQ EKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDT21重组蛋白的3篇参考文献示例(注：内容为模拟概括，具体文献需根据实际检索调整)：
1. **文献名称**: "Structural basis of NUDT21 for recognition of polyadenylation signals"
**作者**: Gruber, A.J., et al.
**摘要**: 该研究解析了重组NUDT21蛋白的晶体结构，揭示了其通过保守结构域识别mRNA前体的聚腺苷酸化信号序列(如AAUAAA)，并阐明了其在3'端加工复合体中的分子机制。
2. **文献名称**: "NUDT21 regulates HIV-1 RNA splicing by recruiting viral transcripts to nuclear speckles"
**作者**: Zhang, Y., et al.
**摘要**: 通过体外重组NUDT21蛋白实验，发现其通过结合HIV-1 RNA的特定序列调控病毒RNA剪接，并证明其功能缺失会导致病毒颗粒生成减少，为抗病毒治疗提供新靶点。
3. **文献名称**: "NUDT21 suppression promotes glioblastoma growth by enhancing EGFR mRNA stability"
**作者**: Yang, X., et al.
**摘要**: 利用重组NUDT21蛋白进行体外结合实验，发现其通过结合EGFR mRNA的3'UTR抑制降解，证明NUDT21在胶质母细胞瘤中通过调控致癌基因稳定性影响肿瘤进展。
建议通过PubMed或Google Scholar检索关键词“NUDT21 recombinant protein”或“CPSF5 recombinant”获取具体文献。
NUDT21 (nudix hydrolase 21), also known as cleavage and polyadenylation specificity factor subunit 5 (CPSF5), is a critical protein involved in RNA metabolism and post-transcriptional regulation. It belongs to the Nudix family of hydrolases, which are characterized by a conserved catalytic motif (GX5EX7REUXEEXGU, where U represents a hydrophobic residue) and function in hydrolyzing substrates like nucleotides or RNA. NUDT21 specifically plays a central role in pre-mRNA 3ʹ-end processing by interacting with the cleavage and polyadenylation specificity factor (CPSF) complex. This process is essential for mRNA maturation, enabling proper nuclear export, stability, and translation.
Recombinant NUDT21 proteins are engineered versions expressed in vitro, typically in bacterial (e.g., E. coli) or mammalian systems, to study its biochemical properties and molecular interactions. Structurally, the protein contains a catalytic Nudix domain and a CPSF25/59/73 domain, facilitating RNA binding and cleavage activity. Research has highlighted its role in alternative polyadenylation (APA), a mechanism that diversifies mRNA isoforms and regulates gene expression in development, cancer, and neurological disorders. Dysregulation of NUDT21 is linked to diseases such as glioblastoma, prostate cancer, and autism spectrum disorders, making it a potential therapeutic target.
The recombinant form enables detailed studies on its enzymatic kinetics, RNA substrate specificity, and interactions with partners like CPSF subunits or RNA molecules. Techniques like X-ray crystallography and cryo-EM using recombinant NUDT21 have provided insights into its 3D structure and mechanism. Additionally, it serves as a tool for drug screening to identify inhibitors modulating APA pathways. Overall, recombinant NUDT21 is vital for deciphering RNA processing mechanisms and their implications in health and disease.
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