| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT2 |
| Uniprot No | P50583 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMALRACGLIIFRRCLIPKVDNNAIEFLLLQ ASDGIHHWTPPKGHVEPGEDDLETALRETQEEAGIEAGQLTIIEGFKREL NYVARNKPKTVIYWLAEVKDYDVEIRLSHEHQAYRWLGLEEACQLAQFKE MKAALQEGHQFLCSIEA |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDT2重组蛋白的3篇文献及其摘要概括(文献信息为虚拟示例，供参考)：
1. **文献名称**：*"Recombinant human NUDT2: Cloning, expression, and biochemical characterization"*
**作者**：Smith A et al.
**摘要**：该研究在大肠杆菌中成功表达并纯化了重组人源NUDT2蛋白，通过酶动力学实验证明其具有水解Ap4A(二腺苷四磷酸)的活性，并分析了金属离子对酶活性的影响。
2. **文献名称**：*"Structural insights into NUDT2-mediated mRNA decapping and its role in cancer"*
**作者**：Li X et al.
**摘要**：通过X射线晶体学解析了重组NUDT2蛋白的三维结构，揭示了其与mRNA帽子结构结合的机制，并发现NUDT2在乳腺癌细胞中通过调控mRNA稳定性影响肿瘤增殖。
3. **文献名称**：*"Functional analysis of NUDT2 in nucleotide metabolism using a yeast expression system"*
**作者**：Yamamoto K et al.
**摘要**：利用酵母系统表达重组NUDT2.证明其参与细胞内Ap4A的代谢调控，并发现其缺失会导致核苷酸池失衡，提示其在维持细胞稳态中的关键作用。
如需真实文献，建议在PubMed或Web of Science中检索关键词“NUDT2 recombinant”或“NUDT2 expression”。
NUDT2 (nucleoside diphosphate-linked moiety X-type motif 2), also known as diadenosine tetraphosphatase, is a member of the Nudix hydrolase family characterized by a conserved catalytic motif (GX5EX7REUXEEXGU, where U = hydrophobic residue). This enzyme primarily catalyzes the hydrolysis of diadenosine polyphosphates, particularly Ap4A (diadenosine tetraphosphate), into ADP and ATP. Ap4A, a "danger signal" molecule accumulating under cellular stress, plays roles in DNA repair, apoptosis regulation, and stress response pathways. NUDT2's activity helps maintain nucleotide pool homeostasis by regulating intracellular Ap4A levels, thereby influencing cellular adaptation to oxidative stress, metabolic fluctuations, and genotoxic challenges.
Recombinant NUDT2 protein is typically produced using bacterial (e.g., E. coli) or eukaryotic expression systems, often fused with affinity tags (His-tag, GST) for purification. Its molecular weight ranges between 18-22 kDa depending on the expression construct. Structural studies using recombinant protein reveal a conserved α/β Nudix fold with a flexible loop involved in substrate binding. The enzyme requires divalent cations (Mg²⁺/Mn²⁺) for catalytic activity, with optimal function at neutral pH.
Research applications include: 1) Investigating nucleotide metabolism dysregulation in cancers (e.g., NUDT2 overexpression in breast cancer correlates with poor prognosis); 2) Studying neurodegenerative diseases where abnormal Ap4A accumulation occurs; 3) Screening small-molecule inhibitors for therapeutic development. Recent studies suggest NUDT2's potential as a biomarker for chemotherapy response and its emerging role in mRNA cap hydrolysis, expanding its relevance in post-transcriptional regulation. Challenges remain in fully elucidating its substrate spectrum and physiological partners across different tissues.
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