| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT16L1 |
| Uniprot No | Q9BRJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSTAAVPELKQISRVEAMRLGPGWSHSCHA MLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGGFVDRRFWSLEDGLNR VLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAVEISAV HSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVL NMMPEEKLVEALAAATEKQKKALEKLLPASS |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDT16L1重组蛋白的构造示例参考文献(内容基于公开研究主题的合理推测，非真实文献)：
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1. **文献名称**: *Structural and Functional Characterization of Human NUDT16L1 as a mRNA Decapping Enzyme*
**作者**: Smith J. et al.
**摘要**: 本研究解析了重组NUDT16L1蛋白的晶体结构，证明其通过水解mRNA 5'端帽结构(如m7GpppN)调控mRNA稳定性，并揭示其底物特异性与细胞应激反应中的潜在作用。
2. **文献名称**: *NUDT16L1 in Hematopoiesis: A Recombinant Protein-Based Study*
**作者**: Lee S. & Chen X.
**摘要**: 利用重组NUDT16L1蛋白进行功能实验，发现其通过清除细胞内的氧化损伤核苷酸(如8-oxo-dGDP)维持造血干细胞的基因组稳定性，提示其在血液疾病中的治疗潜力。
3. **文献名称**: *Role of NUDT16L1 in Viral RNA Degradation: Mechanistic Insights*
**作者**: Gonzalez R. et al.
**摘要**: 通过体外重组蛋白实验证实NUDT16L1可降解含5'端帽的病毒RNA(如流感病毒)，提出其作为天然抗病毒防御机制的一部分，可能影响宿主免疫应答路径。
4. **文献名称**: *NUDT16L1 Dysregulation in Cancer: Recombinant Protein Interaction Analysis*
**作者**: Wang Y. et al.
**摘要**: 研究重组NUDT16L1与肿瘤抑制蛋白p53的相互作用，发现其通过调控特定mRNA代谢影响细胞周期进程，为癌症靶向治疗提供新分子靶点。
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注：以上文献为示例性构造，实际研究中建议通过PubMed或Web of Science以关键词"NUDT16L1 recombinant"查询真实发表论文。
**Background of NUDT16L1 Recombinant Protein**
NUDT16L1 (Nudix Hydrolase 16-Like 1), also known as X29 or DXO, is a member of the NUDIX phosphohydrolase superfamily, characterized by a conserved catalytic motif (GX₅EX₇REUXEEXGU) that enables hydrolysis of nucleotide substrates. This enzyme plays a critical role in RNA metabolism and quality control. It exhibits decapping activity for incompletely capped mRNAs, removing non-canonical 5′-end moieties (e.g., NAD⁺, FAD, or UDP) to prevent their aberrant translation or degradation by exonucleases like XRN1. NUDT16L1 also acts as a surveillance factor for viral RNA, selectively targeting RNAs lacking standard 5′-cap structures, thereby linking it to innate immunity.
Recombinant NUDT16L1 protein is engineered for in vitro studies to elucidate its structural and functional properties. Its crystal structure reveals a conserved NUDIX domain and unique flexible loops that mediate substrate recognition. Research highlights its involvement in cellular stress responses, neurodegenerative disorders, and cancer, where dysregulated RNA processing contributes to pathogenesis. Recombinant forms enable biochemical assays (e.g., enzymatic activity profiling), inhibitor screening, and exploration of its role in viral RNA evasion mechanisms.
Studies using recombinant NUDT16L1 have advanced understanding of RNA quality control pathways and potential therapeutic targeting, particularly in diseases associated with RNA dysregulation. Its dual role in cellular homeostasis and antiviral defense underscores its biological significance.
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