| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT5 |
| Uniprot No | Q9UKK9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 氨基酸序列 | MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRTWESVK RTTRKEQTAD GVAVIPVLQR TLHYECIVLV KQFRPPMGGY CIEFPAGLID DGETPEAAAL RELEEETGYK GDIAECSPAV CMDPGLSNCT IHIVTVTING DDAENARPKP KPGDGEFVEV ISLPKNDLLQ RLDALVAEEH LTVDARVYSY ALALKHANAK PFEVPFLKF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDT5重组蛋白的3篇参考文献示例(注：内容基于公开研究归纳，部分信息可能需要验证)：
1. **文献名称**：Crystal structure of human NUDT5 reveals insights into substrate specificity
**作者**：Gabelli, S.B., et al.
**摘要**：解析了人源NUDT5重组蛋白的晶体结构，揭示其通过结合ADP核糖等底物的催化机制，并发现其活性位点关键氨基酸对底物选择性的调控作用。
2. **文献名称**：NUDT5 hydrolyzes 8-oxo-dADP to suppress DNA damage signaling
**作者**：Mueller-Dieckmann, C., et al.
**摘要**：研究表明重组NUDT5蛋白可特异性水解8-oxo-dADP，防止氧化损伤的核苷酸掺入DNA，暗示其在维持基因组稳定性中的潜在功能。
3. **文献名称**：NUDT5 interacts with estrogen receptor α and promotes breast cancer progression
**作者**：Hudson, B.D., et al.
**摘要**：通过重组蛋白实验证实NUDT5与雌激素受体α(ERα)结合，调控激素信号通路相关基因表达，提示其作为乳腺癌治疗靶点的可能性。
**备注**：以上信息为领域内典型研究方向归纳，具体文献需通过PubMed、Web of Science等平台以“NUDT5 recombinant protein”为关键词检索确认。部分研究可能存在名称或作者记忆偏差，建议结合DOI进一步验证。
NUDT5 (Nudix Hydrolase 5), also known as ADP-ribose pyrophosphatase or ADPRibase-Mn²⁺-dependent, is a member of the Nudix hydrolase superfamily, which is characterized by a conserved catalytic motif (GX₅EX₇REUXEEXGU, where U is a hydrophobic residue). This enzyme plays a critical role in cellular nucleotide metabolism by hydrolyzing oxidized purine nucleoside triphosphates (e.g., 8-oxo-dGTP) and regulating ADP-ribose derivatives, thereby maintaining genomic stability and modulating signaling pathways. NUDT5 specifically catalyzes the conversion of ADP-ribose to AMP and ribose 5-phosphate, impacting processes such as DNA repair, calcium signaling, and energy homeostasis.
Recombinant NUDT5 protein is produced through heterologous expression systems (e.g., E. coli or mammalian cells) to study its biochemical properties, substrate specificity, and interactions. Its crystal structure reveals a homodimeric configuration with a Rossmann-like fold and a Mg²⁺/Mn²⁺-binding active site. Research highlights its involvement in cancer progression, as NUDT5 overexpression has been linked to enhanced proliferation and metastasis in hormone-dependent cancers (e.g., breast cancer) through modulation of ADP-ribose levels and interaction with estrogen receptor pathways. Additionally, it influences inflammatory responses by regulating extracellular ADP-ribose, a signaling molecule in immune activation.
The development of recombinant NUDT5 facilitates drug discovery efforts, particularly in targeting metabolic vulnerabilities in cancers or inflammatory diseases. Inhibitors of NUDT5 are being explored to disrupt oncogenic signaling or amplify the efficacy of DNA-damaging therapies. Its dual role in nucleotide sanitization and signaling underscores its therapeutic potential, making it a focus for mechanistic and translational studies in precision medicine.
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