| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUDT4 |
| Uniprot No | Q9NZJ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMMKFKPN QTRTYDREGF KKRAACLCFR SEQEDEVLLV SSSRYPDQWI VPGGGMEPEE EPGGAAVREV YEEAGVKGKL GRLLGIFENQ DRKHRTYVYV LTVTEILEDW EDSVNIGRKR EWFKVEDAIK VLQCHKPVHA EYLEKLKLGC SPANGNSTVP SLPDNNALFV TAAQTSGLPS SVR |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUDT4重组蛋白的3篇参考文献示例(注：以下内容为虚构示例，实际文献需根据具体数据库查询)：
1. **文献名称**：*"Recombinant Human NUDT4 Protein: Expression, Purification, and Substrate Specificity Analysis"*
**作者**：Sakumi K. et al.
**摘要**：本研究成功在大肠杆菌中重组表达了人源NUDT4蛋白，并通过亲和层析纯化获得高纯度蛋白。实验表明，重组NUDT4对8-oxo-dGDP具有显著水解活性，提示其在修复氧化损伤核苷酸中的潜在作用。
2. **文献名称**：*"Structural Insights into NUDT4 Catalytic Mechanism by Crystallography of Recombinant Protein"*
**作者**：Lee S.J. et al.
**摘要**：通过解析重组NUDT4蛋白的晶体结构，揭示了其活性位点关键氨基酸残基(如Asp80和Glu82)与底物的结合模式，为设计针对NUDT4功能调控的小分子化合物提供了结构基础。
3. **文献名称**：*"Functional Characterization of Recombinant NUDT4 in Cancer Cell Proliferation"*
**作者**：Chen L. et al.
**摘要**：利用HEK293细胞表达重组NUDT4蛋白，发现其过表达显著抑制肿瘤细胞的增殖能力，可能与调节细胞内嘌呤代谢平衡及减少DNA氧化损伤有关。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“NUDT4 recombinant protein”或“NUDT4 expression”。
**Background of NUDT4 Recombinant Protein**
NUDT4 (nucleoside diphosphate-linked moiety X-type motif 4), also known as DIPP1 or hDIPP2. is a member of the NUDIX hydrolase superfamily, a group of enzymes characterized by their conserved NUDIX motif (GX₅EX₇REUXEEXGU, where U = hydrophobic residue). These enzymes primarily hydrolyze nucleoside diphosphates linked to other moieties, playing roles in cellular metabolism, signaling, and homeostasis. NUDT4 specifically catalyzes the degradation of dinucleotide polyphosphates, such as diadenosine hexaphosphate (Ap₆A), and inositol pyrophosphates (e.g., InsP₇/InsP₈), which are critical regulators of energy sensing, phosphate homeostasis, and cellular stress responses.
Functionally, NUDT4 is implicated in modulating mTORC1 (mechanistic target of rapamycin complex 1) signaling by regulating inositol phosphate metabolism, thereby influencing cell growth, proliferation, and autophagy. Dysregulation of NUDT4 has been associated with pathologies including cancer, neurodegenerative disorders, and metabolic syndromes, highlighting its therapeutic potential.
Recombinant NUDT4 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and enzymatic activity. Its production enables detailed biochemical studies, such as substrate specificity assays, inhibitor screening, and structural analyses (e.g., X-ray crystallography), to elucidate molecular mechanisms. Furthermore, recombinant NUDT4 serves as a tool for investigating cellular pathways linked to phosphate signaling and nucleotide homeostasis, as well as for developing targeted therapies against diseases driven by inositol pyrophosphate dysregulation.
In summary, NUDT4 recombinant protein bridges fundamental research and translational applications, offering insights into its biological roles and therapeutic relevance.
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