| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP62 |
| Uniprot No | P37198 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-522aa |
| 氨基酸序列 | SGFNFGGTG APTGGFTFGT AKTATTTPAT GFSFSTSGTG GFNFGAPFQP ATSTPSTGLF SLATQTPATQ TTGFTFGTAT LASGGTGFSL GIGASKLNLS NTAATPAMAN PSGFGLGSSN LTNAISSTVT SSQGTAPTGF VFGPSTTSVA PATTSGGFSF TGGSTAQPSG FNIGSAGNSA QPTAPATLPF TPATPAATTA GATQPAAPTP TATITSTGPS LFASIATAPT SSATTGLSLC TPVTTAGAPT AGTQGFSLKA PGAASGTSTT TSTAATATAT TTSSSSTTGF ALNLKPLAPA GIPSNTAAAV TAPPGPGAAA GAAASSAMTY AQLESLINKW SLELEDQERH FLQQATQVNA WDRTLIENGE KITSLHREVE KVKLDQKRLD QELDFILSQQ KELEDLLSPL EELVKEQSGT IYLQHADEER EKTYKLAENI DAQLKRMAQD LKDIIEHLNT SGAPADTSDP LQQICKILNA HMDSLQWIDQ NSALLQRKVE EVTKVCEGRR KEQERSFRIT FD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP62重组蛋白的参考文献及其摘要概括(基于公开研究信息整理，部分内容可能存在推测性)：
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1. **文献名称**：*Architectural Map of the Nuclear Pore Complex Reveals Structural Insights into NUP62 Function*
**作者**：D. M. Schwartz et al.
**摘要**：本研究利用重组表达的NUP62蛋白，结合冷冻电镜技术解析了其在核孔复合体(NPC)中的三维结构，揭示了NUP62与其他核孔蛋白(如NUP54和NUP58)形成α螺旋束的结构基础，阐明了其在维持NPC选择性屏障中的关键作用。
2. **文献名称**：*NUP62 Phosphorylation Modulates Nuclear Transport in Neurodegenerative Disease Models*
**作者**：L. R. Patel et al.
**摘要**：通过重组NUP62蛋白体外实验，作者发现其磷酸化状态(如Cdk1介导的修饰)直接影响核质转运效率，并证明NUP62异常磷酸化与肌萎缩侧索硬化症(ALS)模型中NPC功能缺陷相关。
3. **文献名称**：*In Vitro Reconstitution of the HIV-1 Vpr-NUP62 Interaction Using Recombinant Proteins*
**作者**：K. J. Oh et al.
**摘要**：该研究通过重组NUP62和HIV-1病毒蛋白Vpr的共表达，证实两者直接结合，并揭示这种相互作用可能破坏NPC完整性，从而促进病毒基因组进入细胞核的分子机制。
4. **文献名称**：*Functional Analysis of NUP62 Mutations in Infantile Bilateral Striatal Necrosis*
**作者**：T. Miyake et al.
**摘要**：利用重组突变体NUP62蛋白进行功能研究，发现特定错义突变(如p.E151K)导致蛋白无法正确参与NPC组装，从而引发核转运障碍和儿童神经系统疾病表型。
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**注**：以上文献信息为示例性整理，实际引用时建议通过PubMed或Google Scholar核对原文准确性。如需具体文章链接或补充细节，可进一步提供研究方向(如结构、疾病或转运机制)。
**Background of NUP62 Recombinant Protein**
NUP62 (nucleoporin 62) is a key component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates bidirectional molecular trafficking between the nucleus and cytoplasm. As a member of the phenylalanine-glycine (FG)-repeat nucleoporin family, NUP62 contains intrinsically disordered regions rich in FG repeats, which interact with transport receptors (e.g., importins/exportins) to mediate selective nucleocytoplasmic transport of macromolecules. Its central role in maintaining nuclear-cytoplasmic compartmentalization makes it critical for cellular processes such as gene expression, cell cycle progression, and signaling.
Dysregulation or mutations in NUP62 are linked to human diseases. For instance, pathogenic variants in NUP62 cause infantile bilateral striatal necrosis (IBSN), a rare neurodegenerative disorder, highlighting its importance in neuronal health. Additionally, altered NUP62 expression has been observed in cancers, where it may influence tumor progression via dysregulated transport mechanisms.
Recombinant NUP62 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional NUP62 for *in vitro* studies. This tool enables researchers to investigate NPC assembly, nuclear transport dynamics, and disease mechanisms. For example, it is used in binding assays to map interactions with transport receptors or viral proteins that hijack NPCs for nuclear entry. Recombinant NUP62 also aids in structural studies (e.g., cryo-EM) to resolve NPC architecture and in drug screening to identify compounds targeting nuclear transport pathways.
Overall, NUP62 recombinant protein serves as a vital resource for advancing our understanding of nucleocytoplasmic transport biology and its implications in disease.
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