| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | D12; P31; SLT1; MDS032 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于USE1抗体的参考文献示例(注：部分信息为模拟概括，建议通过学术数据库核实)：
1. **文献名称**: "Characterization of USE1 in the Ubiquitin Conjugation Pathway"
**作者**: Lee, J. et al.
**摘要**: 本研究验证了USE1抗体在检测内源性UBA6-specific E2酶中的特异性，证实USE1通过调控泛素化过程参与内质网相关降解(ERAD)通路，Western blot及免疫荧光结果显示其在哺乳动物细胞中的定位。
2. **文献名称**: "USE1 Antibody Validation in Neuronal Protein Aggregation Models"
**作者**: Smith, R. & Patel, K.
**摘要**: 文章报道了一种高特异性USE1抗体的开发，并用于研究阿尔茨海默病模型中USE1与错误折叠蛋白的相互作用，证明其通过泛素-蛋白酶体系统调控神经元蛋白稳态。
3. **文献名称**: "Role of USE1 in DNA Damage Response: Insights from Knockdown and Antibody-based Assays"
**作者**: Zhang, Y. et al.
**摘要**: 利用USE1抗体进行免疫共沉淀实验，发现USE1与BRCA1复合物存在相互作用，提示其在DNA损伤修复中的潜在功能，研究为泛素化机制与癌症关联提供了新证据。
4. **文献名称**: "Commercial USE1 Antibody Comparison for Functional Studies"
**作者**: Gomez, L. et al.
**摘要**: 系统性评估了市售多种USE1抗体的灵敏度和交叉反应性，推荐特定品牌抗体用于不同实验场景(如流式细胞术、组织切片染色)，并指出常见使用误区。
**提示**：以上为模拟示例，实际文献建议通过PubMed或Google Scholar以“USE1 antibody”、“UBE2Z antibody”(USE1别名)等关键词检索，并优先选择近5年、高影响因子期刊的研究。
The USE1 antibody targets the UBA6-specific E2-conjugating enzyme 1 (USE1), a key component of the ubiquitination pathway. Ubiquitination regulates protein degradation, signaling, and cellular homeostasis through a cascade involving E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. USE1. as an E2 enzyme, uniquely pairs with UBA6 (an E1 enzyme) to transfer ubiquitin to substrates, distinguishing it from other E2s that rely on the more common UBA1. This specificity highlights USE1's role in selective protein turnover and non-canonical ubiquitination processes.
USE1 is implicated in diverse cellular functions, including endoplasmic reticulum-associated degradation (ERAD), cell cycle regulation, and stress responses. Dysregulation of USE1 has been linked to diseases such as cancer and neurodegenerative disorders. Antibodies against USE1 are critical tools for studying its expression, localization, and interactions in these contexts. They enable techniques like Western blotting, immunofluorescence, and co-immunoprecipitation, aiding in the exploration of USE1's mechanistic contributions to ubiquitination pathways and disease mechanisms.
Research utilizing USE1 antibodies has advanced understanding of UBA6/USE1-specific substrate targeting, offering insights into therapeutic strategies for ubiquitination-related pathologies. These antibodies remain essential for dissecting the complexity of the ubiquitin-proteasome system.
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