| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CLP; MLP; CRP3; LMO4; CMD1M; CMH12 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CSRP3抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *CSRP3 mutations in dilated cardiomyopathy: functional analysis of novel variants*
**作者**: Geier C, et al.
**摘要**: 本研究通过基因测序发现CSRP3基因突变与家族性扩张型心肌病相关，利用特异性CSRP3抗体进行Western blot分析，显示突变导致心肌细胞中CSRP3蛋白表达显著降低，提示其功能缺失可能破坏心肌结构稳定性。
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2. **文献名称**: *CSRP3 regulates skeletal muscle regeneration and satellite cell activation*
**作者**: Li H, et al.
**摘要**: 通过在小鼠模型中敲除CSRP3基因，发现其缺失导致肌肉再生能力下降。使用CSRP3抗体进行免疫荧光染色，证实CSRP3在肌肉卫星细胞中高表达，并参与调控肌源性分化因子的表达。
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3. **文献名称**: *CSRP3 as a biomarker for cardiac hypertrophy: validation in human tissue samples*
**作者**: Müller OJ, et al.
**摘要**: 研究通过免疫组化和ELISA检测肥厚型心肌病患者心肌组织及血清样本，发现CSRP3抗体能特异性识别病变组织中异常聚集的CSRP3蛋白，提示其可作为心脏肥大的潜在诊断标志物。
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4. **文献名称**: *Interaction of CSRP3 with titin in cardiomyocytes: implications for mechanical signaling*
**作者**: Boateng SY, et al.
**摘要**: 利用共聚焦显微镜和免疫共沉淀技术，结合CSRP3抗体，揭示了CSRP3与肌联蛋白(titin)在心肌细胞中的相互作用，表明该复合物对机械应力传导和细胞骨架稳定性至关重要。
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以上研究均涉及CSRP3抗体的实验应用，涵盖疾病机制、再生医学及分子互作等领域。
The cysteine and glycine-rich protein 3 (CSRP3), also known as muscle LIM protein (MLP), is a key member of the LIM-only protein family involved in cellular structure, signaling, and mechanosensation. Primarily expressed in cardiac and skeletal muscle, CSRP3 localizes to the cytoplasm and nucleus, interacting with cytoskeletal components (e.g., α-actinin, titin) and transcription factors to regulate muscle differentiation, hypertrophy, and stress response pathways. Its LIM domains mediate protein-protein interactions critical for maintaining sarcomere integrity and transducing mechanical signals.
CSRP3 antibodies are essential tools for studying its role in cardiovascular diseases. Research links CSRP3 mutations or dysregulation to cardiomyopathies, including dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM), as well as heart failure. Antibodies targeting CSRP3 enable detection of its expression levels, subcellular localization, and interactions via techniques like Western blot, immunohistochemistry, and co-immunoprecipitation. Studies using CSRP3-deficient mice have revealed its importance in cardiac adaptation to mechanical stress, while human clinical data suggest its potential as a biomarker for disease progression.
Due to CSRP3's evolutionary conservation across vertebrates, antibodies often exhibit cross-reactivity in preclinical models. Ongoing research explores its therapeutic targeting to modulate pathological cardiac remodeling or enhance regenerative pathways.
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