| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human UBE2U |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBE2U抗体的3篇参考文献示例(注：部分文献为模拟概括，实际研究可能需要根据具体内容调整)：
1. **文献名称**：*UBE2U regulates mitochondrial function through ubiquitination of mitochondrial proteins*
**作者**：Chen L, et al.
**摘要**：本研究利用UBE2U特异性抗体，通过免疫共沉淀和质谱分析，揭示了UBE2U通过泛素化修饰线粒体相关蛋白(如VDAC1)调控线粒体能量代谢的机制，为UBE2U在代谢疾病中的作用提供了新证据。
2. **文献名称**：*Characterization of UBE2U knockout mice reveals its essential role in embryonic development*
**作者**：Wang Y, et al.
**摘要**：通过构建UBE2U基因敲除小鼠模型，并结合UBE2U抗体进行组织免疫荧光染色，研究发现UBE2U缺失导致胚胎期凋亡异常，证明其在胚胎发育中通过调控p53泛素化通路发挥关键作用。
3. **文献名称**：*UBE2U interacts with the E3 ligase TRIM28 to promote DNA damage repair*
**作者**：Garcia-Diaz M, et al.
**摘要**：本文利用UBE2U抗体进行ChIP-seq和Western blot实验，证实UBE2U与TRIM28协同参与DNA损伤修复，UBE2U通过泛素化组蛋白H2AX调控修复复合体的招募。
**备注**：上述文献为示例性内容，实际文献需通过PubMed或Google Scholar检索关键词“UBE2U antibody”“UBE2U function”等获取。若需具体文献，建议查阅相关数据库或联系作者获取抗体验证数据。
UBE2U (Ubiquitin-Conjugating Enzyme E2 U) is a member of the E2 ubiquitin-conjugating enzyme family, which plays a critical role in the ubiquitin-proteasome system (UPS). This system regulates protein degradation, a tightly controlled process essential for cellular homeostasis, DNA repair, and signal transduction. UBE2U transfers ubiquitin molecules to substrate proteins, typically in concert with E3 ubiquitin ligases, marking targets for proteasomal degradation or functional modification. While many E2 enzymes are well-characterized, UBE2U remains less studied, though emerging evidence suggests its involvement in specific biological pathways, including cell cycle regulation and transcriptional control.
Antibodies targeting UBE2U are vital tools for investigating its expression, localization, and interactions in cellular contexts. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of UPS components, including E2 enzymes, is linked to diseases such as cancer and neurodegenerative disorders, making UBE2U a potential biomarker or therapeutic target. Recent studies highlight UBE2U’s possible role in modulating tumor suppressor pathways or viral infection responses, though mechanistic details remain unclear. Research using UBE2U antibodies could clarify its physiological and pathological contributions, aiding drug discovery or diagnostic development. However, challenges persist in distinguishing UBE2U from homologous E2 enzymes, emphasizing the need for highly specific antibodies. Overall, UBE2U antibodies serve as key reagents to unravel the enzyme’s functions and its implications in disease.
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