首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Apelin酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APLN含量。APLN即Apelin，其前体蛋白经水解可产生多种活性肽。它与G蛋白偶联受体APJ结合，分布于心血管、中枢神经等多个系统。在调节心血管功能、能量代谢、水盐平衡等方面有重要作用，是治疗心血管疾病、肥胖等疾病潜在靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础研究中Apelin在疾病模型中的表达动态分析、药物干预效果评估或信号通路机制探索。为心血管代谢性疾病相关研究提供灵敏可靠的工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apln ELISA kit; ApelApelin ELISA kit; APJ endogenous ligand) [Cleaved into: Apelin-36; Apelin-31; Apelin-28; Apelin-13] ELISA kit
缩写：APLN
Uniprot No.：Q9R0R4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse apelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of mouse apelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-109 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.3872.2722.3302.216 10001.7211.8321.7771.663 5001.2731.2391.2561.142 2500.7090.7430.7260.612 1250.4120.4410.4270.313 62.50.2640.2780.2710.157 31.250.2050.2140.2100.096 00.1160.1120.114 | ||||||
货期：3-5 working days
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