首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白H(Apo-H)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOH含量。APOH即载脂蛋白H，又称β2-糖蛋白 I。它参与细胞内吞、凝血调节等生理过程。研究发现，APOH和自身免疫病、心血管疾病等密切相关，其通过与多种配体相互作用，影响信号传导和免疫反应，是疾病机制和药物研发的潜在靶点。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于小鼠模型中的脂代谢研究、自身免疫性疾病机制探索或心血管相关实验的样本分析；助力于脂蛋白功能研究、疾病动物模型构建及药物干预效果评价等基础科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoh ELISA kit; B2gp1Beta-2-glycoprotein 1 ELISA kit; APC inhibitor ELISA kit; Activated protein C-binding protein ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
缩写：APOH
Uniprot No.：Q01339
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 88 | ||||
| Range % | 83-92 | |||||
| 1:2000 | Average % | 97 | ||||
| Range % | 91-101 | |||||
| 1:4000 | Average % | 89 | ||||
| Range % | 84-92 | |||||
| 1:8000 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of mouse Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-95 | ||||
| EDTA plasma (n=4) | 103 | 98-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.6692.6252.6472.512 5002.1802.1122.1462.011 2501.6111.6001.6061.471 1250.8590.8310.8450.710 62.50.5150.5270.5210.386 31.20.3210.3330.3270.192 15.60.2480.2420.2450.110 00.1360.1340.135 | ||||||
货期：3-5 working days
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