| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | gs129; C16orf73 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MEIOB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MEIOB抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*MEIOB exhibits single-stranded DNA-binding and exonuclease functions in meiotic recombination*
**作者**：Luo M. et al.
**摘要**：该研究通过开发特异性MEIOB抗体，发现MEIOB蛋白在减数分裂中结合单链DNA并具有核酸酶活性，参与交叉重组形成，抗体用于免疫荧光证实其与SIX6OS1的共定位。
2. **文献名称**：*MEIOB is essential for spermatogenesis and forms a complex with SPATA22 in mammals*
**作者**：Souquet B. et al.
**摘要**：利用MEIOB抗体在小鼠睾丸组织中发现MEIOB与SPATA22相互作用，调控减数分裂联会复合体组装，抗体通过免疫沉淀验证蛋白互作网络。
3. **文献名称**：*The meiotic protein MEIOB recruits RPA to coordinate DNA replication and recombination in meiosis*
**作者**：Li X. et al.
**摘要**：研究通过Western blot和免疫荧光(使用MEIOB抗体)揭示MEIOB在减数分裂早期招募RPA蛋白，协调DNA复制与重组，抗体实验显示其在生殖细胞中的动态表达模式。
注：以上文献为示例，实际引用需根据具体研究内容核对原文。
The MEIOB antibody is a crucial tool in studying meiosis and germ cell development. MEIOB (Meiosis Specific with OB-Fold) is a protein predominantly expressed in germ cells, where it plays a vital role in meiotic recombination and DNA repair. It interacts with components like SPATA22 and RPA to form a complex essential for processing recombination intermediates, ensuring accurate homologous chromosome pairing and crossover formation. MEIOB's OB-fold domain facilitates single-stranded DNA binding, critical for stabilizing recombination sites and resolving DNA breaks.
Research using MEIOB antibodies has revealed its localization to meiotic chromosomes, highlighting its involvement in synapsis and crossover regulation. Mutations or deficiencies in MEIOB are linked to gametogenesis defects, impaired fertility, and conditions like non-obstructive azoospermia in humans. These antibodies enable detection of MEIOB expression patterns, protein-protein interactions, and dynamic changes during meiosis, aiding investigations into infertility mechanisms and genome stability.
Developed for applications such as immunofluorescence, Western blotting, and immunohistochemistry, MEIOB antibodies help dissect molecular pathways underlying reproductive health and genetic disorders. Their use in model organisms (e.g., mice) has further elucidated conserved meiotic functions, emphasizing MEIOB's evolutionary significance in sexual reproduction.
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