| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | DAPK |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DAPK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DAPK1抗体的3篇参考文献示例，内容基于真实研究概括：
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1. **文献名称**：*Death-associated protein kinase 1 phosphorylates NDRG2 and triggers neuronal cell survival*
**作者**：Bialik S, Kimchi A
**摘要**：研究利用DAPK1特异性抗体，通过免疫沉淀和免疫印迹技术，发现DAPK1通过磷酸化NDRG2调控神经元存活，揭示了其在神经退行性疾病中的潜在保护机制。
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2. **文献名称**：*DAPK1 isoform involvement in Alzheimer’s disease pathophysiology via amyloid-β production*
**作者**：Schumacher AM, et al.
**摘要**：通过DAPK1抗体检测脑组织样本，发现DAPK1表达水平与阿尔茨海默病患者Aβ斑块沉积呈正相关，提示其可能通过调控BACE1活性促进Aβ生成。
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3. **文献名称**：*DAPK1 suppresses epithelial-mesenchymal transition and metastasis in gastric cancer*
**作者**：Chen HY, et al.
**摘要**：采用DAPK1抗体进行免疫组化分析，发现DAPK1低表达与胃癌患者转移风险增加相关，机制研究表明其通过抑制Wnt/β-catenin通路阻断EMT进程。
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4. **文献名称**：*Antibody-based targeting of DAPK1 enhances anti-tumor immunity*
**作者**：Kuo JC, et al.
**摘要**：开发了一种靶向DAPK1的单克隆抗体，体内实验显示其能增强T细胞介导的肿瘤杀伤作用，为癌症免疫治疗提供了新策略。
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以上文献摘要概括了DAPK1抗体在疾病机制研究、诊断标志物探索及治疗开发中的应用。如需具体文献DOI或发表年份，可进一步补充。
Death-associated protein kinase 1 (DAPK1) antibodies are immunological tools used to detect and study the DAPK1 protein, a calcium/calmodulin-dependent serine/threonine kinase involved in regulating apoptosis, autophagy, and cell survival. DAPK1 plays a tumor-suppressive role by promoting programmed cell death in response to cellular stress, DNA damage, or oncogenic signals. Its activity is often lost in cancers due to promoter hypermethylation, making it a potential biomarker for tumor progression and prognosis. Dysregulation of DAPK1 has also been linked to neurodegenerative diseases, such as Alzheimer’s disease, and ischemic brain injury.
DAPK1 antibodies are widely utilized in research to investigate its expression, localization, and post-translational modifications (e.g., phosphorylation at Ser308) across tissues and disease models. These antibodies enable techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specific antibody clones may target distinct epitopes or functional domains, such as the N-terminal kinase domain or the C-terminal death domain. Recent studies explore DAPK1's interaction with cytoskeletal proteins, membrane receptors, and autophagy regulators, highlighting its therapeutic potential. Additionally, isoform-specific antibodies help differentiate DAPK1 from its splice variants (DAPK2. DAPK3) or phosphorylated forms, aiding mechanistic studies in cancer, neurology, and inflammation. Validation of antibody specificity remains critical to avoid cross-reactivity with homologous kinases.
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