Rabbit Polyclonal CBFB Antibody

The CBFB (Core Binding Factor Beta) antibody is a crucial tool in studying the CBFB protein, a key component of the core-binding factor (CBF) complex. CBFB heterodimerizes with RUNX family transcription factors (e.g., RUNX1) to regulate hematopoietic differentiation, cell proliferation, and apoptosis. This complex binds to DNA through RUNX proteins, while CBFB stabilizes the interaction and enhances DNA-binding affinity. Dysregulation of CBFB, particularly through chromosomal abnormalities like inv(16)(p13q22) or t(16;16), is strongly associated with acute myeloid leukemia (AML), especially the M4Eo subtype, where CBFB fuses with MYH11 to form an oncogenic fusion protein. CBFB antibodies are widely used in research to investigate leukemogenesis mechanisms, diagnose AML subtypes, and assess CBFB expression or fusion proteins in clinical samples. They also aid in studying normal hematopoiesis, transcriptional regulation, and developmental biology. Commercial CBFB antibodies (e.g., clone H-7. 8D12) are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Recent studies explore their potential in monitoring minimal residual disease or therapeutic responses. However, challenges remain in distinguishing wild-type CBFB from fusion variants and optimizing detection specificity across diverse experimental models.