首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子骨碱性磷酸酶(BALP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的BALP含量。BALP即骨碱性磷酸酶，它是成骨细胞的表型标志物。在骨代谢中，成骨细胞分泌BALP参与骨基质的合成与矿化。研究通过检测其水平来反映成骨细胞活性及骨形成状况，在骨疾病如骨质疏松、佝偻病等的诊断与病情监测中有重要意义。试剂盒检测范围为0.156 mIU/mL-10 mIU/mL，本试剂盒适用于骨代谢相关动物模型研究，如骨质疏松症机制探索、骨折愈合进程监测、骨再生药物疗效评估等科研场景，为体外定量分析提供稳定可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：BALP
Uniprot No.：G1SLU6
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：0.156 mIU/mL-10 mIU/mL
灵敏度：0.039 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 81-92 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of rabbit BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 95-108 | ||||
| EDTA plasma (n=4) | 99 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected102.1362.0872.1122.00951.8391.7831.8111.7082.51.5131.4881.5011.3981.251.0791.0281.0540.9510.6250.7520.7220.7370.6340.3120.5620.5540.5580.4550.1560.3350.3270.3310.22800.1020.1040.103 | ||||||
货期：3-5 working days
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