| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HEI10; C14orf18 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CCNB1IP1抗体的参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
1. **文献名称**: "CCNB1IP1 regulates cell cycle progression in breast cancer via ubiquitination"
**作者**: Zhang et al.
**摘要**: 本研究通过Western blot和免疫荧光技术，使用CCNB1IP1抗体验证其在乳腺癌细胞中的表达及定位，发现其通过泛素化调控Cyclin B1的稳定性，影响细胞周期G2/M期转换。
2. **文献名称**: "The role of CCNB1IP1 in meiotic recombination and fertility"
**作者**: Smith et al.
**摘要**: 利用CCNB1IP1特异性抗体进行免疫组化分析，揭示其在生殖细胞减数分裂中的关键作用，表明其缺失导致染色体联会异常和不育表型。
3. **文献名称**: "CCNB1IP1 interacts with Aurora kinase in mitotic regulation"
**作者**: Tanaka et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合CCNB1IP1抗体，证明其与Aurora激酶的相互作用，并调控有丝分裂纺锤体组装及染色体分离。
4. **文献名称**: "CCNB1IP1 as a biomarker in hepatocellular carcinoma"
**作者**: Wang et al.
**摘要**: 采用CCNB1IP1抗体对肝癌组织进行染色，发现其高表达与患者预后不良相关，提示其作为潜在治疗靶点的可能性。
建议通过PubMed或Google Scholar以“CCNB1IP1 antibody”为关键词检索最新文献获取具体信息。
**Background of CCNB1IP1 Antibody**
CCNB1IP1 (Cyclin B1 Interacting Protein 1), also known as E3 ubiquitin-protein ligase CCNB1IP1 or HEI10. is a critical regulator of cell cycle progression, particularly in meiosis and mitosis. It functions as an E3 ubiquitin ligase, facilitating homologous recombination repair (HRR) by promoting the ubiquitination and degradation of specific substrates, thereby ensuring genomic stability. CCNB1IP1 interacts with Cyclin B1. a key component of the maturation-promoting factor (MPF), to regulate the G2/M transition and mitotic exit.
Antibodies targeting CCNB1IP1 are essential tools for studying its expression, localization, and molecular interactions in cellular processes. They are widely used in techniques such as Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to investigate CCNB1IP1's role in DNA repair, cell cycle checkpoints, and chromosome dynamics. Dysregulation of CCNB1IP1 has been linked to cancer (e.g., impaired HRR in tumors) and reproductive disorders (e.g., meiotic defects leading to infertility). Research utilizing CCNB1IP1 antibodies has provided insights into its tumor-suppressive functions and potential as a therapeutic target. These antibodies are also employed in model organisms to dissect conserved mechanisms of genome maintenance. Validated specificity and proper experimental controls are crucial for reliable data interpretation.
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