| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与CCDC12抗体相关的模拟参考文献(基于研究领域常见方向虚构，供参考格式)：
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1. **文献名称**: *Characterization of a Novel Polyclonal Antibody Against Human CCDC12 for Detecting Cytoplasmic Localization in Cancer Cells*
**作者**: Li, X.; Wang, Y.; Zhang, H.
**摘要**: 本研究开发了一种针对人源CCDC12蛋白的多克隆抗体，验证了其在免疫组化(IHC)和免疫印迹(WB)中的特异性，发现CCDC12在乳腺癌细胞质中高表达，并与细胞迁移相关。
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2. **文献名称**: *CCDC12 Interaction with Microtubule Networks: Insights from Immunofluorescence Staining Using a Commercial Antibody*
**作者**: Müller, S.; Braun, R.
**摘要**: 利用市售CCDC12抗体进行免疫荧光实验，发现CCDC12与微管结构共定位，提示其在细胞分裂中可能参与纺锤体组装调控，为纤毛功能障碍疾病研究提供线索。
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3. **文献名称**: *Development of a Monoclonal Antibody Targeting CCDC12 for Proteomic Analysis of Primary Cilia Components*
**作者**: Gupta, A.; Patel, K.
**摘要**: 报道一种高特异性CCDC12单克隆抗体的制备，通过质谱分析证实CCDC12是初级纤毛的关键组分，可能影响Hedgehog信号通路活性。
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注：以上文献为模拟示例，实际研究中请通过PubMed或SciHub等平台检索真实文献。
The CCDC12 (Coiled-Coil Domain Containing Protein 12) antibody is a tool used to study the function and expression of the CCDC12 protein, a member of the coiled-coil domain-containing protein family. These proteins are characterized by their α-helical coiled-coil domains, which mediate protein-protein interactions and structural organization within cells. CCDC12 is evolutionarily conserved and expressed in various tissues, though its precise biological roles remain under investigation. Preliminary studies suggest its involvement in cellular processes such as mitosis, ciliogenesis, and intracellular transport, potentially linked to microtubule-associated functions.
Research using CCDC12 antibodies has helped localize the protein to subcellular structures like the centrosome and cilia, implicating it in ciliary assembly and maintenance. Dysregulation of ciliary proteins is associated with ciliopathies, developmental disorders, and cancers, positioning CCDC12 as a protein of interest in these contexts. Antibodies against CCDC12 enable detection via techniques like Western blot, immunofluorescence, and immunohistochemistry, aiding in expression profiling across normal and diseased tissues.
Current challenges include clarifying CCDC12's molecular mechanisms and validating its interactions with signaling pathways. Its potential role in cancer is emerging, with some studies noting altered expression in tumors. Further work is needed to explore its diagnostic or therapeutic relevance. The CCDC12 antibody thus serves as a critical reagent for unraveling the protein's contributions to cellular physiology and disease.
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