| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CDC45L; MGORS7; CDC45L2; PORC-PI-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CDC45 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDC45抗体的3篇模拟参考文献及其摘要概括(注：文献为示例性质，非真实存在)：
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1. **文献名称**: *CDC45 as a Prognostic Biomarker in Colorectal Cancer: Immunohistochemical Analysis*
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫组化(IHC)分析结直肠癌组织中CDC45蛋白表达水平，发现其高表达与患者不良预后显著相关，提示CDC45可能作为潜在的肿瘤标志物。
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2. **文献名称**: *CDC45 Antibody Validation in DNA Replication Studies Using Western Blot and siRNA Knockdown*
**作者**: Tanaka K, et al.
**摘要**: 该文献报道了一种特异性识别CDC45蛋白的单克隆抗体的开发与验证，通过Western blot和siRNA敲低实验证实其在DNA复制研究中的应用价值，并揭示CDC45在细胞周期S期中的关键作用。
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3. **文献名称**: *Role of CDC45 in HPV-Associated Cervical Cancer: Co-Expression Analysis with Viral Oncoproteins*
**作者**: Chen L, et al.
**摘要**: 研究利用免疫荧光和流式细胞术探讨CDC45与人乳头瘤病毒(HPV)致癌蛋白E6/E7的协同作用，发现CDC45抗体在宫颈癌组织中的检测可辅助评估肿瘤进展及病毒整合机制。
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**备注**：以上文献为示例性内容，实际引用需根据真实发表的论文调整。建议通过PubMed、Google Scholar等平台以“CDC45 antibody”或“CDC45 cancer biomarker”为关键词检索最新研究。
The CDC45 antibody is a crucial tool in studying the role of Cell Division Cycle 45 (CDC45), a conserved protein essential for DNA replication and cell cycle progression. CDC45 forms part of the CMG (CDC45-MCM-GINS) helicase complex, which unwinds DNA during replication initiation in eukaryotes. It interacts with minichromosome maintenance (MCM) proteins and GINS components to ensure proper replication fork movement and genome stability.
Research using CDC45 antibodies has elucidated its involvement in cell cycle checkpoints, replication stress responses, and developmental processes. These antibodies enable detection of CDC45 expression levels, localization, and interactions via techniques like Western blot, immunofluorescence, and co-immunoprecipitation. Dysregulation of CDC45 is linked to diseases, particularly cancers, where its overexpression correlates with uncontrolled proliferation and poor prognosis in malignancies such as breast, liver, and colorectal cancer. Additionally, mutations in CDC45 are associated with developmental disorders like Meier-Gorlin syndrome.
Antibody specificity is validated using knockout cell lines or siRNA-mediated knockdown to confirm target recognition. Commercial CDC45 antibodies are typically raised against conserved epitopes in human, mouse, or rat homologs, with applications spanning basic research and clinical studies. Ongoing investigations focus on CDC45's potential as a biomarker or therapeutic target in oncology, emphasizing its critical role in replication licensing and cancer pathogenesis.
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