关键信息
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基因名
Deoxyhypusine synthase/DHS
- 应用
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别名
MIG13; DHS; DS; Migration-Inducing Gene 13
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种属
Mouse
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q3TXU5
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表达区间
Met1~Asp369
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分子量
44kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
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背景信息
Deoxyhypusine synthase (DHS) is an essential enzyme that catalyzes the first step in the hypusine biosynthesis pathway, specifically the conversion of the translation factor eIF5A's lysine residue to deoxyhypusine. This post-translational modification is crucial for eIF5A's role in protein synthesis and cellular proliferation. Recent research has highlighted DHS as a potential target for cancer therapy, as its activity is often upregulated in various malignancies, contributing to uncontrolled cell growth. The recombinant production of DHS allows for in-depth structural and functional studies, aiding in the understanding of its enzymatic mechanism and its regulatory processes. Investigating the properties of DHS in a recombinant form not only facilitates the exploration of its interactions with substrates and inhibitors but also supports the development of potent pharmacological agents aimed at modulating its activity. By elucidating the role of DHS in cellular physiology and its implications in disease contexts, researchers aim to harness this knowledge for therapeutic advances, particularly in cancer treatments, where selective inhibition of DHS could potentially lead to diminished tumor growth and improved patient outcomes. The ongoing studies on recombinant DHS underscore its significance in both basic research and drug development, making it a pivotal focus within the fields of biochemistry, molecular biology, and medical science.
