首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠B细胞淋巴瘤因子2(Bcl-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的BCL2含量。BCL2是一种抑制细胞凋亡的基因，其表达异常与多种恶性肿瘤的发生发展密切相关。研究机制表明，BCL2通过调节细胞凋亡信号通路，影响肿瘤细胞的生存和增殖。靶向BCL2及其相关蛋白，可抑制肿瘤细胞的生长，为肿瘤治疗提供新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探究小鼠模型中Bcl-2表达与疾病机制的关联，如肿瘤微环境研究、药物干预效果评估或细胞凋亡信号通路分析等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bcl2 ELISA Kit; Bcl-2 ELISA Kit; Apoptosis regulator Bcl-2 ELISA Kit
缩写：BCL2
Uniprot No.：P10417
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Bcl-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 96 | ||||
| Range % | 85-102 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 85-102 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 82-103 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 81-99 | |||||
回收率：
| The recovery of mouse Bcl-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 85-100 | ||||
| EDTA plasma (n=4) | 96 | 87-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10001.9522.0522.0021.838 5001.5271.5911.5591.395 2501.0511.1121.0820.918 1250.6280.5920.6100.446 62.50.4350.4570.4460.282 31.20.2930.3040.2990.135 15.60.2150.2060.2110.047 00.1660.1620.164 | ||||||
货期：3-5 working days
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