首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：人BCL2相关蛋白A1(BCL2A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BCL2A1含量。BCL2A1是重要靶点。它属BCL-2蛋白家族，在细胞凋亡调控中起关键作用。异常表达与肿瘤发生、发展及耐药相关。研究聚焦其作用机制，探索其在肿瘤信号通路里的角色，期望通过靶向它开发新型抗癌疗法。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，基于特异性抗体对靶蛋白的双重识别原理，通过标准品浓度梯度建立标准曲线实现精准定量，适用于细胞凋亡机制研究、肿瘤生物学模型分析、免疫调节实验等科研领域，为探索BCL2A1在疾病发生发展中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACC 1 ELISA Kit; ACC 2 ELISA Kit; B2LA1_HUMAN ELISA Kit; Bcl 2 related protein A1 ELISA Kit; Bcl-2-like protein 5 ELISA Kit; Bcl-2-related protein A1 ELISA Kit; BCL2 related protein A1 ELISA Kit; Bcl2-L-5 ELISA Kit; BCL2A1 ELISA Kit; BCL2L5 ELISA Kit; BFL1 ELISA Kit; GRS ELISA Kit; HBPA1 ELISA Kit; Hematopoietic BCL2 related protein A1 ELISA Kit; Hemopoietic specific early response protein ELISA Kit; Hemopoietic-specific early response protein ELISA Kit; Protein BFL 1 ELISA Kit; Protein BFL-1 ELISA Kit; Protein GRS ELISA Kit
缩写：BCL2A1
Uniprot No.：Q16548
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BCL2A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human BCL2A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 97 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1181.9522.0351.84510001.4261.3381.3821.1925000.8940.8540.8740.6842500.5920.5810.5870.3971250.4550.4380.4470.25762.50.3730.3650.3690.17931.250.2530.2500.2520.06200.1900.1890.190 | ||||||
货期：3-5 working days
×
