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产品描述：猪脑源性神经营养因子(BDNF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BDNF含量。BDNF即脑源性神经营养因子，在神经系统发育和维持中扮演关键角色。研究显示，它能促进神经元存活、生长和分化，增强突触可塑性。其机制是与特定受体结合，激活细胞内信号通路，进而调控基因表达，影响神经系统功能。试剂盒检测范围为9.375 ng/mL-600 ng/mL，猪模型研究中神经退行性疾病机制探索、药物干预效果评估或应激状态下神经营养因子动态变化分析等科研场景，以及神经科学、畜牧兽医及比较医学领域的实验研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BDNFBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
缩写：BDNF
Uniprot No.：P14082
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：9.375 ng/mL-600 ng/mL
灵敏度：2.35 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 91-102 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 109 | |||||
| Range % | 105-113 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 96-107 | ||||||
回收率：
| The recovery of pig BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 98-105 | |||||
| EDTA plasma (n=4) | 86 | 93-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected6002.1342.0812.1082.0163001.5371.6231.5801.4881501.1671.1231.1451.053750.7690.7740.7720.68037.50.5130.5030.5080.41618.750.3530.3440.3490.2579.3750.2270.2210.2240.13200.0910.0930.092 | |||||||
货期：3-5 working days
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